用RAPD和ISSR法研究新疆红花主栽品种的遗传多样性

【目的】对29份新疆红花栽培品种的遗传多样性进行检测。【方法】RAPD和ISSR分子标记技术。【结果】20个RAPD引物和18个ISSR引物分别扩增出156和112条带,多态条带比率(PPB)分别为92.31%和93.77%。RAPD和ISSR检测的有效等位基因数分别为1.508 1和1.513 7,基因多样性为0.3115和0.3416,Shannon多样性指数为0.4738和0.4798。以Nei氏遗传距离矩阵按UPGMA方法聚类分析结果RAPD和ISSR标记的遗传距离分别为0.108 2~2.0541和0.123 4~2.1535。【结论】红花不同品种之间具有比较丰富的遗传变异。对比RAPD和ISSR在PCR反应中的稳定性和检测变异的能力表明,对于实验条件的稳定性而言ISSR优于RAPD,且总的来说ISSR能检测到比RAPD更多的遗传变异。Mantel检测表明:这两种标记的分析结果有极显著的相关性r=0.963。

Genetic Diversity of Carthamus tinctorius L.in Xinjiang Based on ISSR and RAPD Analysis

Objective and Method]The genetic diversity of 29 Carthamus tinctorius L.cultivated varieties in Xinjiang were tested with RAPD and ISSR techniques.Result]156 and 112 DNA bands were amplified with 20 RAPD and 18 ISSR primers respectively,and the percentage of polymorphic bands (PPB) was 92.31% and 93.77% respectively. Effective number of alleles Ne,Nei's index H ,the Shannon information index were 1.508 1 and 1.513 7,0.311 5 and 0.341 6,0.473 8 and 0.479 8 respectively.The UPGMA clustering indicated that the genetic distance of RAPD and ISSR were 0.108 2～2.054 1 and 0.123 4～2.153 5 .Conclusion]These above results showed that there was relatively rich genetic variations in safflower. Comparison between the two marker systems showed that ISSR was better than RAPD in terms of reproducibility and ability of detecting genetic polymorphism.Mantel test revealed that the analyzed result of the two marker had significant correlation r=0.963.