| 高效液相色谱法测定豆芽中2,4-D含量 |
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| 引用本文： | 闵季春,陈石金.高效液相色谱法测定豆芽中2,4-D含量[J].现代农业科技,2014(10):282-282. |
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| 作者姓名： | 闵季春 陈石金 |
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| 作者单位： | 江苏省金湖县农业技术推广中心;金湖县农产品质量检测中心; |
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| 摘 要： | 使用高效液相色谱法检测豆芽中2,4-D残留量,用氢氧化钠溶液作为提取剂,采用超声波法提取样品中的2,4-D,经SPE净化,采用紫外检测器检测;用保留时间进行定性分析,外标法进行定量计算。结果表明:标准曲线为Y=4 024.187 3x-113.999 9,相关系数0.9999,线性范围为0.05～10.00μg/mL,方法检出限为0.02 mg/kg,回收率为90.3%～104.7%,相对标准偏差为0.52%～11.18%。
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| 关 键 词： | 高效液相 豆芽 2 4-D 测定 |
Determination of 2.4-D Residue in Bean Sprouts by HPLC |
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| Institution: | MIN Ji-chun, CHEN Shi-jin (1 Agro-Tech Extension Center of Jinhu County in Jiangsu Province, Jinhu Jiangsu 211600; 2 Agricultural Products Quality Test Center of Jinhu County) |
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| Abstract: | Sodium hydrate was used as extracting agent,the ultrasonicwave method was used to extract 2,4-D in bean sprouts.The samples were purified by SPE and detected by UV detector.RT was used in qualitative analysis,and external standard method was used in quantitative calculation. The results showed that the standard curve was Y=4 024.187 3x-113.999 9,the correlation coefficient was 0.999 9,the linearity range was 0.05～10.00μg/mL,the detection limit of method was 0.02 mg/kg,the standard recovery was 90.3%～104.7%,and RSD was 0.52%～11.18%. |
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| Keywords: | HPLC bean sprouts 2 4-D determination |
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