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RAPD Analysis of Twelve General Species of Gladiolus hybridus Hort.
作者姓名:WANG  Jingang  ZHAO  Hongkun  GONG  Shufang  CHE  Daidi
作者单位:1. College of Horticulture,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC; 2. Jilin Academy of Agricultural Science,Gongzhuling Jilin 136100,PRC
摘    要:Gladiolus hybridus Hort., also named Chang or- chid, gladiola, Shi Yang jin and Flat Zhulian, is a perennial bulb plant of Iridaceae Gladiolus and one of the famous four cutting flowers in the world. Gladiolus hybridus Hort. is an exotic species. There is…

关 键 词:唐菖蒲  切花  品种  RAPD  遗传多态性
文章编号:1006-8104(2006)-02-0112-04
收稿时间:2006-01-04

RAPD Analysis of Twelve General Species of Gladiolus hybridus Hort
WANG Jingang ZHAO Hongkun GONG Shufang CHE Daidi.RAPD Analysis of Twelve General Species of Gladiolus hybridus Hort.[J].Journal of Northeast Agricultural University,2006,13(2):112-115.
Authors:WANG Jingang  ZHAO Hongkun  GONG Shufang  CHE Daidi
Abstract:RAPD analysis was applied in studying the classification and genetic relationship of 12 cultivars of Gladiolus hybridus Hort. Twenty-six arbitrary primers screened from 80 primers were used for further PCR and diversity analysis. A total of 164 RAPD sites were detected with a mean of 6.31 fragments amplified for each primer. A total of 151 polymorphic DNA fragments were detected among all the 164 amplified fragments, which accounted for a high level of 92.8% of all and could be used for identification of different cultivars. The result revealed that the germplasm resource of Gladiolus hybridus Hort. cultivar had a narrow genetic base on molecular level.
Keywords:Gladiolus hybridus Hort    RAPD  polymorphism
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