葡萄属植物种质资源遗传多样性的RAPD分析

以起源于中国的野葡萄12个种23个株系、欧美杂种6个品种、河岸葡萄3份、15个欧洲葡萄品种共47份材料为试材,采用RAPD技术对葡萄属植物种质资源遗传多样性进行研究。从520个随机引物中获得16个多态性好的引物用于RAPD分析,共获得DNA条带258个,其中多态性条带186个,DNA条带大小介于100~3 500 bp之间。应用STATISTICA获得了47份葡萄材料树谱图,供试材料可分为6类。欧洲葡萄、欧美葡萄杂种、河岸葡萄与中国野葡萄亲缘关系较远。在中国野葡萄中,山葡萄与其他种亲缘关系较远,刺葡萄次之。

Genetic Diversity Based on Random Amplified Polymorphic DNA (RAPD) in Vitis

Genetic diversity among 47 genotypes of grape,including a set of 23 genotypes of 12 wild Vitis species native to China,six inter-specific hybrids (V. vinifera ×V. labrusca),three clones of V. riparia,and fifteen V. vinifera L. cultivars,was investigated based on RAPD analysis. Sixteen polymorphic random primers were selected for the further analysis from the screening of 520 oligonucleotide primers. A total of 186 polymorphic RAPD markers among 258 bands,size ranging from 100 bp to 3500 bp,were produced from the 16 selected primers. Relationships among the 47 clones or accessions based on their genetic distances were clustered using unweighted pair-group method arithmetic average (UPGMA) analysis in a dendrogram. Forty-seven grape species were clustered to six groups. The largest distance was found between V. vinifera,V. riparia,inter-specific hybrid (V. vinifera ×V. labrusca) and the wild grapes native to China. Among the wild Vitis species native to China,the largest distance was found between V. amurensis and the other wild species.