| HPLC分离甘肃棘豆中苦马豆素 |
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| 引用本文： | 童德文,曹光荣,赵献军.HPLC分离甘肃棘豆中苦马豆素[J].西北农业学报,2001,10(2):6-8. |
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| 作者姓名： | 童德文 曹光荣 赵献军 |
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| 作者单位： | 西北农林科技大学畜牧兽医学院 |
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| 基金项目： | 国际科学基金(IFS,NO2906-1)和国家自然科学基金(39770571)资助项目 . |
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| 摘 要： | 甲醇浸泡甘肃棘豆粉 ,回收甲醇浸泡液至浸膏 ,1 mol/L HCl溶解后上 73 2型强酸性阳离子交换柱 ,1 mol/L NH4 OH洗脱并挥发至干 ,得到总生物碱。总生物碱经氨性氯仿提取后 ,通过高效液相色谱柱分离 ,色谱条件是 :反相 ( C1 8硅胶 )柱 ,在 2 5min内用 5%～ 1 0 0 %甲醇线性梯度洗脱 ,苦马豆素一般出现在 80 %～1 0 0 %的范围内 ,表现为一个宽峰。高效液相色谱柱分离后回收溶液得到一种白色细针状结晶 1 .0 4 mg,植物中的提取率为 0 .0 0 52 %。经 TLC、MP、IR、MS鉴定分析 ,确定所分离到的结晶为苦马豆素
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| 关 键 词： | 甘肃棘豆 苦马豆素 HPLC |
| 文章编号： | 1004-1389(2001)02-0006-03 |
| 收稿时间： | 2000/2/22 0:00:00 |
| 修稿时间： | 2000年2月22日 |
Isolation and Identification of Swainsonine from O. Kansuensis Bunge |
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| TONG De-wen,CAO Guang-rong and ZHAO Xian-jun.Isolation and Identification of Swainsonine from O. Kansuensis Bunge[J].Acta Agriculturae Boreali-occidentalis Sinica,2001,10(2):6-8. |
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| Authors: | TONG De-wen CAO Guang-rong and ZHAO Xian-jun |
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| Institution: | College of Animal Science and Veterinary Medicine, Northwest Sci Tech University of Agiculture and Forestry, Yangling Shaanxi 712100, China;College of Animal Science and Veterinary Medicine, Northwest Sci Tech University of Agiculture and Forestry, Yangling Shaanxi 712100, China;College of Animal Science and Veterinary Medicine, Northwest Sci Tech University of Agiculture and Forestry, Yangling Shaanxi 712100, China |
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| Abstract: | O.kansuensis Bunge powder was dipped in methanol. The residue following evaporation of the methanol was dissolved in 1 mol/L HCl and applied to a strong cation exchange resin and eluted with 1 mol/L NH 4OH. After extracting the crude alkaloid with ammoniated chloroform, the residue was preparation by HPLC, using reverse phase (C 18 silica) and water/methanol, starting at 5% methanol and a linear gradient to 100% in 25 minutes. 1.04 mg crystal was obtained by HPLC, the yield was 0.0052% based on the plant. The crystal was swainsonine identified by TLC, MP, IR and MS. |
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| Keywords: | O kansuensis Bunge Swainsonine HPLC |
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