| 温敏型亚麻雄性不育系育性相关基因的RAPD初探 |
| |
| 引用本文： | 王利民,党占海,张建平.温敏型亚麻雄性不育系育性相关基因的RAPD初探[J].西北农业学报,2009,18(1):123-127. |
| |
| 作者姓名： | 王利民 党占海 张建平 |
| |
| 作者单位： | 甘肃省农业科学院,作物研究所,甘肃兰州,730070 |
| |
| 基金项目： | 国家自然科学基金，甘肃省自然科学基金 |
| |
| 摘 要： | 通过优化组合,建立了适合本试验的亚麻基因组DNA最佳RAPD反应体系;应用分离群体分组分析法(BSA),利用1S×Argus杂交组合F2分离群体所构建的可育基因池(BF)和不育基因池(BS)进行育性基因的RAPD标记分析.经多次重复试验,在所用的240条10 bp随机引物中筛选出1条多态性引物S1113,其序列为:CACGGCACAA.S1113在可育基因池中扩增出大小约为450 bp的多态性DNA片段,暂命名为S1113450,可能与亚麻温敏雄性不育系可育基因连锁.
|
| 关 键 词： | 亚麻 温敏雄性不育 |
| 收稿时间： | 2008/6/28 0:00:00 |
| 修稿时间： | 2008/7/12 0:00:00 |
Preliminary Screening of RAPD Markers for Fertility Gene of Thermo-sensitivity Male-sterile Line 1S of Flax |
| |
| WANG Limin,DANG Zhanhai and ZHANG Jianping.Preliminary Screening of RAPD Markers for Fertility Gene of Thermo-sensitivity Male-sterile Line 1S of Flax[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(1):123-127. |
| |
| Authors: | WANG Limin DANG Zhanhai and ZHANG Jianping |
| |
| Institution: | Institute of Crop, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;Institute of Crop, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;Institute of Crop, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China |
| |
| Abstract: | With bulked segregant analysis(BSA),bulked male-fertile DNA pool and bulked male-sterile DNA pool were used for RAPD analysis which were respectively consisted of 10 fertile plant individuals and 10 sterile plants from F2 generations of 1S ×Argus. PCR amplification was carried out with 240 10 by primers. After many experiments,the primer 51113(CACGGCACAA)was found that produced polymorphism DNA fragment between two bulked DNA pools. The polymorphism amplificanon fragment (450 bp) appeared in bulked male-sterile DNA pool andpossibly linked to male-fertility gene for the thermo-sensitivity male-sterile line of flax. |
| |
| Keywords: | RAPD |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《西北农业学报》浏览原始摘要信息 |
| 点击此处可从《西北农业学报》下载免费的PDF全文 |
|
