| 枸杞甜菜碱含量测定方法的比较研究 |
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| 引用本文： | 张自萍,郭荣,廖国玲,马佳卉.枸杞甜菜碱含量测定方法的比较研究[J].西北农业学报,2007,16(6):292-295. |
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| 作者姓名： | 张自萍 郭荣 廖国玲 马佳卉 |
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| 作者单位： | 1. 宁夏大学,西部特色生物资源保护与利用教育部重点实验室,宁夏银川,750021
2. 宁夏大学,西部特色生物资源保护与利用教育部重点实验室,宁夏银川,750021;宁夏医学院药检系,宁夏银川,750021 |
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| 基金项目： | 国家科技专项基金;宁夏自然科学基金 |
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| 摘 要： | 通过比较分光光度法和高效液相色谱法测定枸杞甜菜碱含量,以确定适宜的甜菜碱含量测定方法。试验采用甲醇加热回流提取枸杞甜菜碱,分光光度法和NH2柱未经衍生高效液相色谱法分别测定甜菜碱含量。结果表明,NH2柱未经衍生高效液相色谱法快速、简便、重复性好,所测甜菜碱含量明显高于分光光度法。试验认为,NH2柱未经衍生高效液相色谱法能快速准确地测定枸杞甜菜碱含量,可用于枸杞的质量控制。
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| 关 键 词： | 枸杞 甜菜碱 高效液相色谱法 分光光度法 含量测定 |
| 文章编号： | 1004-1389（2007）06-0292-04 |
| 收稿时间： | 2007-04-20 |
| 修稿时间： | 2007-06-28 |
Comparative Study on Methods for Determination of Betaine from Lycium barbarum L. |
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| ZHANG Zi-ping,GUO Rong,LIAO Guo-ling and MA Jia-hui.Comparative Study on Methods for Determination of Betaine from Lycium barbarum L.[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(6):292-295. |
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| Authors: | ZHANG Zi-ping GUO Rong LIAO Guo-ling and MA Jia-hui |
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| Institution: | Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China;Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China;Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China;Pharmacology and Medical Exam Dept. of Ningxia Medical College, Yinchuan 750021, China;Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China |
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| Abstract: | To select the suitable determination spectrophotometry in determining the content method through comparing the methods of HPLC and of betaine from Lycium barbarum L. Methanol extract of Lycium barbarum L. was refluxed and the content of betaine was determined separately by spectrophotometry and HPLC with NH2 stationary phase without derivatization of hetaine. Result showed that the HPLC method is rapid, accurate, and the content of betaine determined by HPLC was higher than spectrophotometry. HPLC with NH2 stationary phase can he applied to determine the content of betaine from L ycium barbarum L. |
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| Keywords: | Lyciumbarbarum L Betaine HPLC Spectrophotometry Determination |
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