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基于体外降糖活性的桑葚渣水提物的超声辅助工艺优化
引用本文:范铭,向露,刘哲,陆胜民.基于体外降糖活性的桑葚渣水提物的超声辅助工艺优化[J].浙江农业学报,2019,31(3):480.
作者姓名:范铭  向露  刘哲  陆胜民
作者单位:1.浙江省农业科学院 食品科学研究所,浙江省果蔬保鲜与加工技术研究重点实验室,农业农村部果品采后处理重点实验室,浙江 杭州 310021; 2.浙江师范大学 化学与生命科学学院,浙江 金华 321004
基金项目:浙江省重点研发计划(2017C02004)
摘    要:以桑葚渣为原料,采用超声辅助提取方法获得水提物并对其α-淀粉酶和α-葡萄糖苷酶的抑制活性进行检测,通过L9(34)正交试验优化了桑葚渣水提物的超声辅助工艺。结果表明:在最佳的提取工艺,即提取时间1.5 h、料液比1∶40(g·mL-1)、超声温度50 ℃、超声功率350 W条件下,提取液对α-淀粉酶和α-葡萄糖苷酶活性的抑制率分别为76.4%、81.4%。

关 键 词:桑葚渣  超声辅助提取  α-淀粉酶抑制活性  α-葡萄糖苷酶抑制活性  工艺优化  
收稿时间:2018-07-02

Optimization of ultrasonic-assisted technology on mulberry residue extracts with in vitro hypoglycemic activity
FAN Ming,XIANG Lu,LIU Zhe,LU Shengmin.Optimization of ultrasonic-assisted technology on mulberry residue extracts with in vitro hypoglycemic activity[J].Acta Agriculturae Zhejiangensis,2019,31(3):480.
Authors:FAN Ming  XIANG Lu  LIU Zhe  LU Shengmin
Institution:1. Key Laboratory of Fruit and Vegetables Postharvest and Processing Technology of Zhejiang Province, Key Laboratory of Post-Harvest Handling of Fruit, Ministry of Agriculture Rural Affairs, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China
Abstract:Mulberry fruit was extracted with deionized water using ultrasonic-assisted method and inhibition rate to alpha-amylase and alpha-glucosidase activities by the extracts was monitored. The extraction process was optimized by L9 (34) orthogonal test. The results showed that, under the optimum conditions, i.e. 1.5 h of extraction time, 1∶40 (g·mL-1) in the ratio of material to liquid, 50 ℃ in ultrasonic temperature and 350 W in ultrasonic power, the inhibition rate of the mulberry fruit extracts to alpha-amylase and alpha-glucosidase was 76.4% and 81.4%, respectively.
Keywords:mulberry fruit  ultrasonic assisted extraction  alpha-amylase activity  alpha-glucosidase activity  process optimization  
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