| 鲮,麦鲮和野鲮之RAPD的遗传标记 |
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| 引用本文： | 郑光明,张跃.鲮,麦鲮和野鲮之RAPD的遗传标记[J].上海水产大学学报,1999,8(3):215-220. |
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| 作者姓名： | 郑光明 张跃 |
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| 作者单位： | 珠江水产研究所农业部热带亚热带鱼类选育与养殖重点开放实验室，广州510380，珠江水产研究所农业部热带亚热带鱼类选育与养殖重点开放实验室，广州510380，珠江水产研究所农业部热带亚热带鱼类选育与养殖重点开放实验室，广州510380，珠江水产研究所农业部热带亚热带鱼类选育与养 |
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| 基金项目： | “九五”国家攻关项目!96－008－01－03－06 |
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| 摘 要： | 以野生和池塘养殖的６４个鲮（Ｃｉｒｒｈｉｎａｍｏｎｌｉｔｏｒｅｌｌａ）１０个麦鲮（Ｃ．ｍｒｉｇｏｌａ）和１０个野棱（Ｌａｂｅｏｒｏｈｉｔａ）样本为材料进行了随机扩增多态性ＤＮＡ（ＲＡＰＤ）遗传分析，通过２６个１０ｂｐ随机引物扩增鲮，麦鲮和野鲮样品分别得到１０７，７３，８７个ＤＮＡ片断，其多态性百分率分别为３５．５％，３９．７％，３７．９％，鲮与野棱，麦鲮与野鲮及鲮与麦鲮的遗传距离分别为０．２２，０
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| 关 键 词： | 鲮 麦鲮 野鲮 随机扩增 遗传标记 RAPD |
THE GENETIC MARKERS OF CIRRHINA MOLITORELLA,C.MRIGOLA AND LABEO ROHIT FROM RAPD |
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| ZHENG Guang-Ming, ZHANG Yue, ZHU Xin-Ping, LUO Jian-Ren, XIA Shi-Ling.THE GENETIC MARKERS OF CIRRHINA MOLITORELLA,C.MRIGOLA AND LABEO ROHIT FROM RAPD[J].Journal of Shanghai Fisheries University,1999,8(3):215-220. |
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| Authors: | ZHENG Guang-Ming ZHANG Yue ZHU Xin-Ping LUO Jian-Ren XIA Shi-Ling |
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| Abstract: | The genetic assessment of 64 wild and cultivated mud carp, Cirrhina molitorella, 10 India mrigal, C. mrigola and 10 India rohu, Label rohita is inferred from its RAPD analysis. 107, 73, 87 DNA fragments in mud carp, India mrigal and India rohu are amplified respectively by 26 10bp-primers. The polymorphic loci percentage is 35. 5%,39. 7%, 37. 9% respectively. The genetic distance of mud carp-India rohu, mud carp-India mrigal and India mrigal-India rohu is 0. 22, 0. 58, 0. 64 respectively. The relationship of mud carp-India rohu is more close than mud carp-India mrigal and India mrigal-India rohu by the dendrogram of the three species based on UPGMA and NJ. This is similar to allozyme analysis, but there is differential from traditional taxonomy. |
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| Keywords: | Cirrhina molitorella Cirrhina mrigola Labeo rohita RAPD genetic marker |
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