| 绥农10号大豆的再生体系研究 |
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| 引用本文： | 李建科,杨静慧,武春霞,刘艳军,王祥,刘秉楠.绥农10号大豆的再生体系研究[J].天津农学院学报,2011,18(1):9-11. |
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| 作者姓名： | 李建科 杨静慧 武春霞 刘艳军 王祥 刘秉楠 |
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| 作者单位： | 1. 天津农学院园艺系,天津,300384;天津中日农村环境资源合作中心,天津,300384
2. 天津农学院园艺系,天津,300384 |
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| 基金项目： | 天津市科委科技支撑项目，1，国家教育部留学基金 |
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| 摘 要： | 为了建立大豆的愈伤组织再生体系,以大豆绥农10号的破坏生长点的茎尖、子叶、真叶、胚轴为外植体,研究了不同激素种类、浓度对大豆愈伤组织形成和芽的再生及生根的影响.结果表明,6-BA对大豆愈伤组织的形成有抑制作用,其中对真叶的抑制作用最大,对破坏生长点的茎尖外植体抑制作用最小:6-BA诱导的大豆芽分化率均较低,其中在2～3...
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| 关 键 词： | 大豆 外植体 激素 再生 芽分化 生根 |
Researches of 'Suinong 10'Soybean Regeneration System |
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| LI Jian-ke,YANG Jing-hui,WU Chun-xia,LIU Yan-jun,WANG Xiang,LIU Bing-nan.Researches of 'Suinong 10'Soybean Regeneration System[J].Journal of Tianjin Agricultural College,2011,18(1):9-11. |
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| Authors: | LI Jian-ke YANG Jing-hui WU Chun-xia LIU Yan-jun WANG Xiang LIU Bing-nan |
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| Institution: | 1(1.Department of Horticulture,Tianjin Agricultural University,Tianjin 300384,China;2.China-Japan(Tianjin)Collaborative Research Center for the Rural Environment and Resource,Tianjin 300384,China) |
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| Abstract: | The regeneration system of soybean was researched by the broken stem top,cotyledons,leaves,hypocotyls of ’Suinong 10’ soybean under the treatment of different hormone for callus,bud and root regeneration.The results show that the callus was restrained by 6-BA and the calluses of broken stem top,hypocotyls and cotyledons were more than one of small leaves and the callus of broken stem top was the most in all explants.The bud differentiation ratio was higher in the KT treatment than that in the 6-BA treatment.The bud regeneration ratio of leaves,epicotyl,hypocotyls was 0 in the 6-BA treatment and the ratio was 4.3%～10.2% with broken stem top by 2～3 mg/L 6-BA.The best culture medium of bud regeneration was MS+0.5 KT with 26.5% of bud regeneration ratio.The root differentiation ratio was the best and reached 96% in the treatment of 1/2 MS+0.5 IBA. |
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| Keywords: | soybean explant hormone bud differentiation rooting |
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