| 诊断超声照射对大鼠早孕胚胎组织细胞Caspase-3表达的影响 |
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| 引用本文： | 刘丽娟,徐晓红,杨永光.诊断超声照射对大鼠早孕胚胎组织细胞Caspase-3表达的影响[J].湛江医学院学报,2013(5):507-508. |
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| 作者姓名： | 刘丽娟 徐晓红 杨永光 |
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| 作者单位： | 广东医学院附属医院超声科;广东医学院附属医院肝胆外科 |
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| 基金项目： | 广东医学院青年基金项目(No.XQ0851) |
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| 摘 要： | 目的 了解诊断超声照射对大鼠早孕胚胎组织细胞Caspase-3表达的影响.方法 40只孕7dSD大鼠随机分为4组,用3.5 MHz超声持续照射腹部子宫体表投影区,分别照射0、10、20、30 min,超声照射后24 h取胚胎组织,用SP免疫组织化学检测Caspase-3蛋白表达.结果 超声照射20、30 mir组,Caspase-3表达明显高于对照组（P＜0.01）.结论 经腹诊断超声持续照射大鼠早孕胚胎超过20 min可使Caspase-3蛋白表达增高,从而可能导致早孕胚胎组织细胞凋亡.
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| 关 键 词： | 超声 Caspase-3 凋亡 大鼠 胚胎 |
Effect of diagnostic ultrasound irradiation on Caspase-3 expression of rat embryo cells during early pregnancy |
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| ,LIU Li-juan;XU Xiao-hong;YANG Yong-guang.Effect of diagnostic ultrasound irradiation on Caspase-3 expression of rat embryo cells during early pregnancy[J].Journal of Guangdong Medical College,2013(5):507-508. |
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| Authors: | ,LIU Li-juan;XU Xiao-hong;YANG Yong-guang |
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| Institution: | 2LIU Li-juan;XU Xiao-hong;YANG Yong-guang;Ultrasound Department,Affiliated Hospital of Guangdong Medical College; |
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| Abstract: | Objective To investigate the effect of diagnostic ultrasound exposure on caspase-3 expression of rat embryo cells during early pregnancy. Methods Forty SD rats at 7 days of pregnancy were randomly divided into 4 groups, and the abdominal projection areas of the uteri were irradiated by 3.5 MHz sonography at 0, 10, 20,30 min. Caspase-3 expression of embryo cells was detected by SP immunohistochemical technique at 24 h after ultrasound exposure. Results Caspase-3 expression of embryo cells increased after 20 and 30 rain exposure compared with the control group （P〈0.01）. Conclusion Caspase-3 protein expression of early embryos can be upregnlated when continuous ultrasound exposure is over 20 min, which might result in apoptosis of rat embryo cells during early pregnancy. |
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| Keywords: | ultrasound caspase-3 apoptosis rat embryo |
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