| 高效液相色谱法及紫外可见分光光度法测定侧柏叶中黄酮含量的比较 |
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| 引用本文： | 丁航,梁统,周克元,揭新明.高效液相色谱法及紫外可见分光光度法测定侧柏叶中黄酮含量的比较[J].湛江医学院学报,2002,20(6):422-423. |
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| 作者姓名： | 丁航 梁统 周克元 揭新明 |
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| 作者单位： | [1]广东医学院生化教研室,广东湛江524023 [2]广东医学院分析中心,广东湛江524023 |
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| 基金项目： | 广东省重点学科基金资助 (GX 93 0 7)，广东省卫生厅基金项目 (A 2 0 0 2 474) |
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| 摘 要： | 目的：通过对高效液相色谱法(HPLC)及紫外可见分光光度法测定例柏叶黄酮进行比较，寻找一种较准确、简单的测定侧柏叶黄酮的方法。方法：HPLC法：流动相由乙睛：0．01mol/L KH2PO4：乙酸(28：72：1．5，V／V)组成；流速1．0mL.min^-1；检测波长为260nm，以槲皮甙为参照品。紫外／可见分光光度法：以芦丁为参照品，410nm波长测定黄酮含量。结果：两种方法都有较好的精密度．但测定的结果有较大差异．紫外／可见分光光度法测得的结果比HPLC法高。结论：以槲皮甙为定量指标更能准确地反映侧柏叶的内在质量，HPLC法是一种较理想的测定方法。
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| 关 键 词： | 高效液相色谱法 紫外可见分光光度法 测定 侧柏叶 黄酮 含量 |
| 文章编号： | 1005-4057(2002)-06-0422-02 |
| 修稿时间： | 2002年2月28日 |
Comparison of HPLC and UV-VIS spectrophotometry for the detection of anthoxanthin in leaves of Platychadus orientalis |
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| DING Hang,LIANG Tong,ZHOU Ke yuan,et al.Comparison of HPLC and UV-VIS spectrophotometry for the detection of anthoxanthin in leaves of Platychadus orientalis[J].Journal of Guangdong Medical College,2002,20(6):422-423. |
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| Authors: | DING Hang LIANG Tong ZHOU Ke yuan |
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| Abstract: | Objective:To find a simple method for the detection of anthoxanthin in leaves of Platychadus orientalis. Methods:Anthoxanthin content was determined by HPLC(velocity:1.0 mL/min;wavelength,260 nm)and UV VIS spectrophotometry(wavelength:410 nm),respectively.The contrast samples were quercitrin for HPLC and rutin for UV VIS spectrophotometry.Results:Both methods had high measurement precision,but the measurement result in UV VIS spectrophotometry was about 3 times that in HPLC.Conclusion:Quercitrin as a quantitative indication can precisely reflect anthoxanthin quality.HPLC may be an ideal method for detection of anthoxanthin in leaves of Platychadus orientalis. |
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| Keywords: | Platychadus orientalis Quercitrin HPLC spectrophotometry |
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