| Plant Male Sterility Induced by Anti-Gene CYP86MFin Brassica oleracea var. Italica |
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| 作者姓名： | HUANG Ke CAO Jia-shu YU Xiao-lin YE Wan-zhi LU Gang XIANG Xun |
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| 作者单位： | [1]Institute of Vegetable Science, Zhejiang University, Hangzhou 310029, P.R.China [2]Research Center of Vegetable Science, Fujian Academy of Agricultural Sciences, Fuzhou 350013, P.R.China |
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| 基金项目： | The study was supported by the National Natural Science Foundation of China （NSFC,30370975） and Zhejiang Province Key Program of Science and Technology （021102536）. |
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| 摘 要： | An anti-gene CYP86MF was introduced into hypocotyls of broccoli （Brassica oleracea L.var. italica Plenck） with Agrobacterium tumefaciens, and the transgenic plants were obtained by kanamycin selection. The results of PCR, Southern blot and Northern blot indicated that the anti-CYP86MF has been integrated into chromosome of the transgenic plant. And also, plants with hypogenetic stamina or ungerminated pollen were observed. The transgenic male sterility plant could fructify via artificial pollination with normal pollen. Thus it was proved that the pistil of male sterility plant was normally developed, and the sterility originated from anti-CYP86MF.
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| 关 键 词： | 芸苔 花椰菜 雄性不育系 抗基因型 |
Plant Male Sterility Induced by Anti-Gene CYP86MFin Brassica oleracea var. Italica |
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| HUANG Ke CAO Jia-shu YU Xiao-lin YE Wan-zhi LU Gang XIANG Xun.Plant Male Sterility Induced by Anti-Gene CYP86MFin Brassica oleracea var. Italica[J].Agricultural Sciences in China,2005,4(11):806-810. |
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| Authors: | HUANG Ke CAO Jia-shu YU Xiao-lin YE Wan-zhi LU Gang XIANG Xun |
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| Abstract: | An anti-gene CYP86MF was introduced into hypocotyls of broccoli (Brassica oleracea L.var. italica Plenck) with Agrobacterium tumefaciens, and the transgenic plants were obtained by kanamycin selection. The results of PCR, Southern blot and Northern blot indicated that the anti-CYP86MF has been integrated into chromosome of the transgenic plant.And also, plants with hypogenetic stamina or ungerminated pollen were observed. The transgenic male sterility plant could fructify via artificial pollination with normal pollen. Thus it was proved that the pistil of male sterility plant was normally developed, and the sterility originated from anti-CYP86MF. |
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| Keywords: | Brassica oleracea Broccoli Anti-gene Male sterility |
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