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Use RAPD Analysis to Classify Tea Trees in Yunnan
Authors:SHAO Wan-fang  PANG Rui-hua  DUAN Hong-xing  WANG Ping-sheng  XU Mei  ZHANG Ya-ping  LI Jia-hua
Abstract:RAPD assessment on genetic variations of 45 tea trees in Yunnan was carried out. Eight primers selected from 40 random primers were used to amplify 45 tea samples, and a total of 95 DNA bands were amplified, of which 90 (94.7 %) were polymorphism. The average number of DNA bands amplified by each primer was 11.5. Based on the results of UPGMA cluster analysis of 95 DNA bands amplified by 8 primers,all the tested materials could be classified into 7 groups including 5 complex groups and 2 simple groups, which was basically identical with morphological classification. In addition, there were some speciations in 2 simple groups.
Keywords:Yunnan  Tea trees  RAPD  Genetic diversity
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