| Genetic Diversity in Wild Populations of Poacynum hendersonii from Inland Arid Regions of Northwest China |
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| 作者单位： | ZHAO Jin-feng1，ZHANG Wei-ming1，2，PENG Xue-mei1，TANG Ze-zi1，GU Gong-ping2，LU Chang-mei1 College of Life Sciences，Nanjing Normal University Nanjing 210046 Nanjing Institute for Comprehensive Utilization of Wild Plants |
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| 摘 要： | Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii.Method] Random amplified polymorphic DNA(RAPD)technique was employed to analyze the genetic diversity in five wild populations of P.hendersonii sampled from Xinjiang,Gansu and Qinghai provinces.Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers,of which 110 were polymorphic,accounting for 66.67%.At species level,Nei's gene diversity index(H),Shannon's information index(I)and genetic differentiation coefficient(Gst)were 0.220 5,0.304 7 and 0.908 2,respectively.P.hendersonii germplasm resources share a high level of genetic diversity,and genetic differentiation mainly exists among the populations.Results from genetic distances and cluster analysis showed that relationships among P.hendersonii populations were to some extent related with their geographical and climatic characters.Conclusion] This study suggests that the conservation of P.hendersonii should focus on the protection of many populations,particularly the Qinghai population.
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Genetic Diversity in Wild Populations of Poacynum hendersonii from Inland Arid Regions of Northwest China |
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| Authors: | ZHAO Jin-feng ZHANG Wei-ming PENG Xue-mei TANG Ze-zi GU Gong-ping LU Chang-mei College of Life Sciences Nanjing Normal University Nanjing Nanjing Institute for Comprehensive Utilization of Wild Plants |
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| Institution: | ZHAO Jin-feng1,ZHANG Wei-ming1,2,PENG Xue-mei1,TANG Ze-zi1,GU Gong-ping2,LU Chang-mei1 College of Life Sciences,Nanjing Normal University Nanjing 210046 Nanjing Institute for Comprehensive Utilization of Wild Plants |
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| Abstract: | Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii.Method] Random amplified polymorphic DNA(RAPD)technique was employed to analyze the genetic diversity in five wild populations of P.hendersonii sampled from Xinjiang,Gansu and Qinghai provinces.Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers,of which 110 were polymorphic,accounting for 66.67%.At species level,Nei's gene diversity index(H),Shannon's information index(I)and genetic differentiation coefficient(Gst)were 0.220 5,0.304 7 and 0.908 2,respectively.P.hendersonii germplasm resources share a high level of genetic diversity,and genetic differentiation mainly exists among the populations.Results from genetic distances and cluster analysis showed that relationships among P.hendersonii populations were to some extent related with their geographical and climatic characters.Conclusion] This study suggests that the conservation of P.hendersonii should focus on the protection of many populations,particularly the Qinghai population. |
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| Keywords: | Poacynum hendersonii Wild population Genetic diversity RAPD |
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