| HPLC法测定光疗药物竹红菌乙素的含量 |
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| 引用本文： | 安红波,阮洪生,张爽,刘荣鑫.HPLC法测定光疗药物竹红菌乙素的含量[J].黑龙江八一农垦大学学报,2009,21(3):70-72. |
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| 作者姓名： | 安红波 阮洪生 张爽 刘荣鑫 |
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| 作者单位： | 黑龙江八一农垦大学生命科学技术学院,大庆,163319 |
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| 基金项目： | 黑龙江八一农垦大学引进硕士科研启动基金 |
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| 摘 要： | 从竹红菌中提取竹红菌甲素，将其转化为竹红菌乙素，建立了HPLC测定合成竹红菌乙素含量的方法。采用Shim-pack VP-ODS色谱柱，色谱条件：柱温为30℃；甲醇-水（体积比83：17）为流动相；紫外检测波长300nm。竹红菌乙素的保留时间为11.5min。竹红菌乙素质量在0.29-1.45μg时，峰面积与质量的线性关系良好（r=0.9993），回收率为98.3％，RSD为1.2％（n=5）。该分析方法简便、快速，对于竹红菌乙素合成过程中转化率测定提供一定的理论依据。
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| 关 键 词： | HPLC法 竹红菌乙素 含量 |
Determination of Phototherapy Medicine Hypocrellin B by HPLC |
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| An Hongbo,Ruan Hongsheng,Zhang Shuang,Liu Rongxin.Determination of Phototherapy Medicine Hypocrellin B by HPLC[J].Journal of Heilongjiang August First Land Reclamation University,2009,21(3):70-72. |
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| Authors: | An Hongbo Ruan Hongsheng Zhang Shuang Liu Rongxin |
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| Institution: | (College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, 163319) |
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| Abstract: | Hypocrellin A was extracted from Hypocrella Bambusesae, then synthesized into Hypocrellin B. HPLC was used for the determination of Hypocrellin B . The suitable chromatogram conditions were as follows: Shim-pack VP-ODS column was as the stationary phase at 30 ℃, the mobile phase was methanol-water (83:17 by volume), and the detection wavelength was 300 nm.The save time of Hypocrellin B was 11.5 min. There was a good linear relationship (r=0.9993) between mass concentration and peak area in the range of 0.29～1.45 μg. The average recovery was 98.3%, and RSD was 1.2% (n=5). The method is simple and quick,therefore can be used for the determination of Hypocrellin B in synthesis. |
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| Keywords: | HPLC Hypocrellin B content |
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