| 柱前衍生化高效液相色谱检测木糖液中葡萄糖含量 |
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| 引用本文： | 贾鹏禹,孙蕊,李良玉,李洪飞,张桂芳,孙大庆,于伟.柱前衍生化高效液相色谱检测木糖液中葡萄糖含量[J].黑龙江八一农垦大学学报,2012,24(3):41-44. |
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| 作者姓名： | 贾鹏禹 孙蕊 李良玉 李洪飞 张桂芳 孙大庆 于伟 |
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| 作者单位： | 1. 黑龙江八一农垦大学黑龙江省农产品加工工程技术研究中心,大庆,163319
2. 黑龙江八一农垦大学动物科技学院 |
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| 摘 要： | 建立了木糖母液及发酵液中葡萄糖的柱前衍生化HPLC检测方法。以l-苯基-3-甲基-5-吡唑啉酮（PMP）为柱前衍生化试剂，在ZORBAXEclipseXDB-C18色谱柱上，流动相采用95％A（醋酸盐缓冲液，O．1mol·L-1，pH=5．71），5％B（乙腈）等比洗脱，检测波长245rim，带宽50nm。目标组分葡萄糖衍生物基线分离无干扰，葡萄糖在2．5-50．0mg·L-1。浓度范围内呈现良好的线性关系，方法检测限为0．2mg·L-1。方法样品前处理简便，灵敏度高，重现性好。
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| 关 键 词： | 木糖液 葡萄糖 1-苯基-3-甲基-5-吡唑啉酮(PMP) 衍生化 HPLC |
Determination of Glucose in Solution of Xylose by Pre-column Derivatization High Performance Liquid Chromatography |
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| Jia Pengyu,Sun Rui,Li Liangyu,Li Hongfei,Zhang Guifang,Sun Daqing,Yu Wei.Determination of Glucose in Solution of Xylose by Pre-column Derivatization High Performance Liquid Chromatography[J].Journal of Heilongjiang August First Land Reclamation University,2012,24(3):41-44. |
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| Authors: | Jia Pengyu Sun Rui Li Liangyu Li Hongfei Zhang Guifang Sun Daqing Yu Wei |
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| Institution: | 1(1.Agri-Food Processing and Engineering Technology Research Center of Heilongjiang Province,Heilongjiang Bayi Agricultural university,Daqing 163319;2.College of Animal Science&Veterinary Medicine,Heilongjiang Bayi Agricultural university) |
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| Abstract: | The determination method of glucose in solution of xylose was established with pre-column derivatization HPLC in this paper.The content of glucose in solution of xylose was determined by RP-HPLC,and pre-column derivatization by 1-phenyl-3-methyl-5-pyrazolone(PMP),and a ZORBAX Eclipse XDB-C18(150 mm×4.6 mm ID,5 μm)column was used,mobile phase was 0.1 mol·L-1 NH4Ac(pH=5.71)and flow rate was 1.0 mL·min-1 and UV detector at a wave length of 245 nm(BW 50 nm).The calibration curve was linear in the mass concentration range of 2.5~50.0 mg·L-1,the LOD of method was 0.20 mg·L-1.The HPLC method is simple,convenient and sensitive. |
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| Keywords: | solution of xylose glucose PMP derivatization HPLC |
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