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二花脸猪FSH β亚基位点PCR-SSCP标记与产仔数关系初探
引用本文:陈杰,姜志华,刘红林,李齐贤,方美英,吴彦宁.二花脸猪FSH β亚基位点PCR-SSCP标记与产仔数关系初探[J].南京农业大学学报,1999,22(2):55-58.
作者姓名:陈杰  姜志华  刘红林  李齐贤  方美英  吴彦宁
作者单位:南京农业大学动物科技学院
基金项目:农业部“九五”畜牧业重点科研计划
摘    要:采用PCR-SSCP(PolymeraseChainReaction-SingleStrandConformationPolymorphism)作为遗传标记,对二花脸猪FSHβ亚基位点与产仔数性状的关系进行了研究。二花脸猪在被扩增位点有较大的变异,而在作为对照品种的大约克猪中则完全纯合。两品种在该位点基因频率差异显著。以标记基因型为因子,分别对二花脸猪群体的头胎产仔数、头三胎产仔数之和以及最高产仔数进行方差分析。结果表明,该标记位点与产仔数性状显著相关,其贡献率达到10%以上。本文还就产仔数指标的选择作了讨论。

关 键 词:二花脸猪  FSH  β亚基  PCR-SSCP  产仔数

Relationship between PCRSSCP at FSH subunit locusand litter size in the Erhualian pigs
Chen Jie,Jiang Zhihua,Liu Honglin,Li Qixian,Fang Meiying and Wu Yanning.Relationship between PCRSSCP at FSH subunit locusand litter size in the Erhualian pigs[J].Journal of Nanjing Agricultural University,1999,22(2):55-58.
Authors:Chen Jie  Jiang Zhihua  Liu Honglin  Li Qixian  Fang Meiying and Wu Yanning
Abstract:The relation between FSH subunit locus and litter size in the Erhualian pigs was studied by means of PCRSSCP in this paper.The Large White pigs were used as a control breed.Various genotypes were found in the Erhualian pigs,while only one type in the Large White pigs.The gene frequency was much different between two breeds at this locus.Variance analysis of litter size at the first parity,the sum of the first three parities and the largest parity was done in various genotypes of the Erhualian pigs.The result indicated that there was high linkage between the PCRSSCP marker and litter size.A discuss on how to select a good index for litter size was given as well.
Keywords:Erhualian pigs  FSH  subunit  PCRSSCP  litter size  
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