| 大豆水溶性蛋白测定方法探讨 |
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| 引用本文： | 刘海顺,张志航,胡瑞丰,马冰雪,牛佳佳,杨兆明.大豆水溶性蛋白测定方法探讨[J].粮食储藏,2011,40(3):48-49,52. |
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| 作者姓名： | 刘海顺 张志航 胡瑞丰 马冰雪 牛佳佳 杨兆明 |
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| 作者单位： | 中央储备粮北京分公司质检中心 067001 |
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| 摘 要： | 对大豆水溶性蛋白测定方法进行探讨,对大豆水溶性蛋白影响因素进行分析,同时,对提取条件进行优化。实验结果表明,温度的影响最为显著（P〈0.05）,所得最优提取完全的条件为：温度35℃,每次加100 mL水,提取2次,每次提取时间为60 min。通过研究后获得的试验条件在操作上较灵活,所提取的水溶蛋白具有更稳定、彻底和准确度高等特点。
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| 关 键 词： | 大豆 水溶性蛋白 测定方法 |
OPTIMIZATION OF DETERMINATION METHOD FOR WATER-SOLUBLE PROTEINS FROM SOYBEAN |
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| Liu Haishun,Zhang Zhihang,Hu Ruifeng,Ma Bingxue,Niu Jiajia,Yang Zhaoming.OPTIMIZATION OF DETERMINATION METHOD FOR WATER-SOLUBLE PROTEINS FROM SOYBEAN[J].Grain Storage,2011,40(3):48-49,52. |
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| Authors: | Liu Haishun Zhang Zhihang Hu Ruifeng Ma Bingxue Niu Jiajia Yang Zhaoming |
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| Institution: | Liu Haishun Zhang Zhihang Hu Ruifeng Ma Bingxue Niu Jiajia Yang Zhaoming(Quality Determination Center of Beijing Grain Storage,State Grain Reserves 067001) |
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| Abstract: | The method for determination of soybean water-soluble proteins was investigated,and the extraction conditions for the solubility were also optimized.The results showed that the effects of temperatures were significant（P 0.05）.The best conditions for extraction were as follows： temperature was 35℃,100 mL of Water was added each time,extraction times were twice,each extraction in 60 min.The test was more convenient,more stable,complete and accurate when the soybean water-soluble proteins was extracted through this method. |
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| Keywords: | soybean water-soluble proteins determination method |
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