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银杏叶提取物对肉仔鸡淋巴细胞增殖及细胞因子水平的影响
引用本文:黄其春,杨小燕,林淑慧,何玉琴,李,焰.银杏叶提取物对肉仔鸡淋巴细胞增殖及细胞因子水平的影响[J].中国农学通报,2012,28(14):23-26.
作者姓名:黄其春  杨小燕  林淑慧  何玉琴    
作者单位:1. 龙岩学院生命科学学院,福建龙岩364000;预防兽医学与生物技术福建省高等学校重点实验室,福建龙岩364000
2. 龙岩学院生命科学学院,福建龙岩364000;福建省人畜寄生与病毒性疫病防控工程技术中心,福建龙岩364000
3. 福建农林大学动物科学学院,福州,350002
基金项目:福建省科技计划重点项目“银杏叶提取物对肉鸡免疫功能影响的研究”(2007N0049)
摘    要:为了研究银杏叶提取物(EGB)对肉仔鸡淋巴细胞增殖及细胞因子水平的影响。将360只1日龄健康AA肉用母雏鸡随机分成5组,每组6个重复,其中一组为对照组,饲喂基础日粮;其余4组分别饲喂添加0.009%金霉素及0.15%、0.20%和0.25%EGB的日粮,试验期为42天。测定肉仔鸡外周血和脾脏淋巴细胞转化率以及血清中细胞因子IL-1、IL-2、IFN-γ、TNF-α的含量。结果显示,日粮中添加不同水平EGB可促进肉仔鸡外周血和脾脏淋巴细胞增殖转化,提高血清中细胞因子IL-1、IL-2、IFN-γ、TNF-α的水平,并且在日粮中添加EGB的作用效果比添加金霉素的作用效果好。表明日粮中添加EGB可增强肉仔鸡的免疫功能。

关 键 词:银杏叶提取物  肉仔鸡  淋巴细胞增殖  细胞因子
收稿时间:2011/11/2 0:00:00
修稿时间:2011/11/21 0:00:00

Effects of Ginkgo Biloba Extract on Lymphocyte Proliferation and the Level of Cytokines in Broiler Chicks
Huang Qichun , Yang Xiaoyan , Lin Shuhui , He Yuqin , Li Yan.Effects of Ginkgo Biloba Extract on Lymphocyte Proliferation and the Level of Cytokines in Broiler Chicks[J].Chinese Agricultural Science Bulletin,2012,28(14):23-26.
Authors:Huang Qichun  Yang Xiaoyan  Lin Shuhui  He Yuqin  Li Yan
Institution:1College of Life Science,Longyan University,Longyan Fujian 364000;2Key Laboratory of Fujian Universities Preventive Veterinary Medicine and Biotechnology,Longyan Fujian 364000;3Fujian Provincial Engineering Technology Center of Prevention and Control in Zoonotic Parasite and Viral Livestock Diseases,Longyan Fujian 364000;4College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002)
Abstract:In order to investigate the effects of ginkgo biloba extract(EGB) on lymphocyte proliferation and the level of cytokines in broiler chicks.Three hundred and sixty one-day-old healthy Arbor Acres female broiler chicks were divided into five groups,each with six replicates.The first group was the control group and fed a basal diet,while the other four groups were fed the same basal diet supplemented with 0.009% aureomycin or 0.15%,0.20%,0.25% EGB.The experiment lasted 42 days.Lymphocyte proliferation of peripheral blood and spleen and the level of cytokine IL-1,IL-2,IFN-γ,TNF-α in serum were determined.The results showed that adding EGB to diets of broilers could improved lymphocyte proliferation of peripheral blood and spleen and increase the level of cytokine IL-1,IL-2,IFN-γ,TNF-α in serum.The effect of adding EGB to diets was better than that of adding aureomycin to diets.The study suggested that adding EGB to diets could enhance the immunity of broilers.
Keywords:ginkgo biloba extract  broiler chicks  lymphocyte proliferation  cytokines
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