大叶黄杨幼茎愈伤组织诱导的研究初报

以大叶黄杨的幼茎段为外植体，在添加BA和NAA、IBA、2,4-D不同激素组合的MS培养基上培养，对其愈伤组织进行诱导试验。结果表明：①生长素对愈伤组织诱导的效应为2,4-D >NAA>IBA；②在不同激素组合培养基上均可诱导出愈伤组织，其中MS+BA1.0~2.0 mg/L+NAA 1.0~1.5 mg/L、MS+BA1.5~2.0 mg/L+IBA1.0~1.5 mg/L、MS+BA0.5~1.0 mg/L+2,4-D 0.5~1.5 mg/L等对愈伤组织的诱导效果最好，其诱导率分别为74.3%、65.3%、81.1%。因此,通过科学配制不同激素组合的MS培养基，就能有效地诱导出大叶黄杨幼茎的愈伤组织。

Preliminary Study on Callus Induction of Tender Stem of Euonymus Japonicus

With young stem segment of Euonymus japonicus as explants, callus induction were studied on MS basal medium supplemented with different hormone combinations including BA and NAA、IBA 、2,4-D. The result indicated that (1) The effect sequence of auxin was 2,4-D > NAA >IBA from the callus induction. (2) Callus were induced on medium of different hormone combinations，and MS+BA1.0~2.0 mg/L+NAA 0.5~1.5 mg/L、 MS+BA1.5~2.0 mg/L+ IBA1.0~1.5mg/L、 MS+BA0.5~1.0 mg/L+2,4-D 0.5~1.5mg/L etc. was the best one for callus induction，and the rate of induction was respectively 74.3%、65.3%、81.1%. So through scientific preparation，callus induction of young stem can be induced effectively，on MS medium with different hormone combinations.