山东大豆育成品种种子萌芽期耐盐性鉴定

盐害是一种重要的非生物胁迫之一,限制植物的生长并降低作物的产量和品质。对9个山东大豆育成品种种子进行不同盐浓度处理(5、10、15 g/L),统计了种子的发芽数、相对发芽率、株高、胚根长和须根数,分析了其相对盐害指数,评价各品种的耐盐性。结果表明:随着盐浓度的增加,品种间的发芽数、株高和须根数都呈下降趋势,品种间存在显著差异。在5 g/L的盐浓度处理下,3个品种的胚根长较对照呈增长趋势,其他指标在3个盐浓度处理下均呈降低趋势。在5 g/L的盐浓度处理下,9个品种都为高耐盐品种;在10 g/L的盐浓度处理下,有5个高耐盐品种;在15 g/L的盐浓度处理下,有1个高耐盐品种。证明了不同盐浓度处理下相对盐害指数与发芽率呈极显著负相关关系,能更好地反映大豆的耐盐性,为大豆耐盐种质创制提供丰富的数据参考和亲本材料,为完善大豆种质资源耐盐性研究和提高盐渍土壤利用效率提供资料依据。

Salt Tolerance of Shandong Soybean Improved Varieties at Germination Stage

Salt damage is one of the important abiotic stresses, limiting plant growth and reducing crop yield and quality. Seeds of 9 Shandong soybean improved varieties were treated with different concentrations of NaCl solution (5, 10, 15 g/L) respectively, and their relative salt injury indexes were analyzed through calculating the seed germination number, relative germination rate, plant height, radicle length and the number of fibrous roots. The results showed that: with the increase of salt concentration, the seed germination number, plant height, and root number all had a decreasing trend, and there were significant differences among the varieties. Under the treatment of 5 g/L salt concentration, the radicle length of 3 varieties showed a rising trend, but other indexes decreased under all the three salt concentrations. Under the treatment of 5 g/L salt concentration, the 9 varieties were all high salt tolerant. Under the treatment of 10 g/L salt concentration, there were 5 hightolerance varieties; under 15 g/L salt concentration, there was one high salt-tolerant variety. Under different salt concentrations, relatively salt injury index had an extremely and significantly negative correlation with the germination rate, well reflecting the salt tolerance of soybean, and providing rich data references and parent materials for improving soybean germplasm resources and the utilization efficiency of saline soil.