鸡源性大肠杆菌产超广谱β-内酰胺酶基因型分布

摘要: 检测鸡大肠杆菌超广谱β-内酰胺酶（ESBLs）的基因型并了解辽宁省鸡源性大肠杆菌产ESBLs基因型分布。对大连4个地区不同鸡场临床分离32株产ESBLs鸡大肠杆菌分别用TEM、OXA、CTX-M和SHV等4种通用引物进行PCR扩增确定其基因型。结果显示32株鸡大肠杆菌均检出TEM型基因、12株检出CTX-M型基因、2株检出OXA型、未检出SHV型；TEM+CTX-M双阳性检出11株、TEM+OXA双阳性检出2株。TEM基因亚型占检出比例最高（100％）。结论：目前辽宁省产ESBLs鸡大肠杆菌基因亚型以TEM为主，其次为CTX-M型，未发现SHV型；以TEM+CTX-M双阳性检出率最高（34.4％）。

Genotype Distribution of Extended-Spectrum β-Lactamases Produced by Escherichia coli Isolated from the Fowl in Dalian Area of Liaoning

To elucidate the genotype distributions of ESBLs produced by E. coli isolated from the fowl in Liaoning Province. Thirty-two ESBL-producing Escherichia coli were collected from four different chicken farms in Dalian, and were analyzed by PCR amplifying using the primers of TEM, OXA, SHV and CTX-M, respectively. The results showed that: thirty-two strains with TEM-type, twelve CTX-M-types and two OXA-types were detected except SHV-type was not found; moreover, eleven strains with multiple TEM + CTX-M and two...