荷斯坦牛DRA基因多态性及序列特征分析

为探讨荷斯坦牛DRA基因的多态性及序列特征,本研究采用基因组DNA混合池扩增并直接测序的方法对荷斯坦牛DRA基因编码区进行多态性分析,同时利用生物信息学方法预测DRA基因的理化特性及其编码蛋白的高级结构。结果表明,荷斯坦牛DRA基因编码区上有4个碱基突变,包含1个错义突变和3个同义突变。DRA基因共编码253个氨基酸,编码产物是一种稳定的不可溶蛋白,具有15个潜在的磷酸化位点,二级结构为混合型,三级结构由α-螺旋、β-折叠、β-转角和无规则卷曲组成。本研究结果为荷斯坦牛的疾病相关基因筛选和抗病分子育种提供了一定的理论依据。

Polymorphism and Sequence Characterization of DRA Gene in Holstein Cattle

In order to analyze the polymorphism and sequence characteristics of DRA gene from Holstein cattle, polymorphism in encoding regions of DRA gene from Holstein cattle was analyzed via pooled genomic DNA amplification and sequencing, the physicochemical characteristics of DRA gene and advanced structures of its coding protein were predicted by bioinformatics methods. The results showed there were 4 SNPs in encoding regions of DRA gene from Holstein cattle, including 1 missense mutation and 3 synonymous mutations. The encoding protein was stable and insoluble containing 253 amino acids and 15 potential phosphorylation sites. The putative secondary structure of DRA protein was mixed and putative protein tertiary structure was consisted of alpha helix, β-pleated sheet, β-turn and random coil. These results could provide theoretical basis for selection of disease correlation genes and molecular breeding for disease resistance in Holstein cattle.