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壳聚糖-胰蛋白酶抑制剂复合可食性膜的制备及抗黄曲霉活性
引用本文:张 宾,汪东风,邓尚贵,林慧敏,唐 艳.壳聚糖-胰蛋白酶抑制剂复合可食性膜的制备及抗黄曲霉活性[J].农业工程学报,2012,28(4):287-292.
作者姓名:张 宾  汪东风  邓尚贵  林慧敏  唐 艳
作者单位:1. 浙江海洋学院食品与药学学院,舟山,316000
2. 中国海洋大学食品科学与工程学院,青岛,266000
基金项目:国家自然基金项目(31071628);国际合作项目(2010DFB34220);浙江省教育厅项目(Y201121697);浙江海洋学院科研启动经费资助(21135011010、21135003411)
摘    要:为探索新型生物膜材料的制备方法及抗黄曲霉活性,以壳聚糖和大豆胰蛋白酶抑制剂(TI)提取物为原料,甘油为增塑剂,利用溶液共混流延法制备壳聚糖-TI-甘油复合可食性膜,测试其厚度、表观结构、力学性质、透光率、水蒸气透过率及抗黄曲霉侵染活性。结果表明,当壳聚糖浓度为18mg/mL、TI浓度2mg/mL、甘油浓度12mg/mL和干燥温度45℃时,制备复合膜具有优良抗黄曲霉活性,且综合理化性能最佳。制备壳聚糖-TI-甘油复合膜液涂膜于花生上,接种黄曲霉培养后发现,复合膜对于黄曲霉侵染具有较强的抵抗和抑制作用。

关 键 词:生物膜  甘油  透光率  黄曲霉毒素  壳聚糖  胰蛋白酶抑制剂  复合膜
收稿时间:2011/3/25 0:00:00
修稿时间:6/7/2011 12:00:00 AM

Preparation and anti-Aspergillus ?avus activity of chitosan-trypsin inhibitor blend edible film
Zhang Bin,Wang Dongfeng,Deng Shanggui,Lin Huimin and Tang Yan.Preparation and anti-Aspergillus ?avus activity of chitosan-trypsin inhibitor blend edible film[J].Transactions of the Chinese Society of Agricultural Engineering,2012,28(4):287-292.
Authors:Zhang Bin  Wang Dongfeng  Deng Shanggui  Lin Huimin and Tang Yan
Institution:1(1. Department of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China;2. Department of Food Science, Ocean University of China, Qingdao 266000, China)
Abstract:In order to explore the preparation method of biological film and its activity of anti-Aspergillus flavus, the chitosan-based blend film was prepared from chitosan, soybean trypsin inhibitor extract (TI) and glycerol solution, and the properties of which were also investigated, including thickness, mechanical property, water vapor transmission, optical transmittance, solubility apparent structure and anti-A. flavus activity. The results showed that the chitosan, TI and glycerol concentration got 18 mg/mL, 2 mg/mL and 12 mg/mL respectively, the blend films exhibited good physical and chemical properties, and the germination and growth of A. flavus were strongly inhibited by blend films on peanuts.
Keywords:biofilms  glycerol  light transmittance  aflatoxins  chitosan  trypsin inhibitor  blend film
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