| 辣椒子叶不定芽的诱导与伸长研究 |
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| 引用本文： | 谢礼洋,匡华琴,赖钟雄,刘生财.辣椒子叶不定芽的诱导与伸长研究[J].黑龙江农业科学,2013(10):12-15. |
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| 作者姓名： | 谢礼洋 匡华琴 赖钟雄 刘生财 |
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| 作者单位： | 福建农林大学园艺植物生物工程研究所,福建福州350002 |
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| 基金项目： | 福建农林大学本科教学改革资助项目（No.111412099） |
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| 摘 要： | 为了进一步研究辣椒子叶再生体系的影响因素，以辣椒王种子为试材，诱导无菌苗，取子叶作为外植体，探讨了不同浓度6-BA对辣椒子叶诱导不定芽的影响，并进行了不同浓度GA3与6-BA组合对不定芽伸长效果的研究。结果表明：辣椒子叶在MB5＋0．8mg·L^-1IAA＋8．0mg·L^-16-BA＋4．0mg·L^-1AgNO3培养基上诱导不定芽效果最好；MB5＋0．8mg·L^-1IAA＋5．0mg·L^-16-BA＋2．0mg·L^-1GA3＋4．0mg·L^-1AgNO3培养基有利于不定芽的伸长。
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| 关 键 词： | 辣椒 子叶 不定芽诱导 伸长 |
Study on Adventitious Buds Induction and Elongation of Capsicum annuum L. Cotyledon |
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| XIE Li-yang,KUANG Hua-qin,LAI Zhong-xiong,LIU Sheng-cai.Study on Adventitious Buds Induction and Elongation of Capsicum annuum L. Cotyledon[J].Heilongjiang Agricultural Science,2013(10):12-15. |
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| Authors: | XIE Li-yang KUANG Hua-qin LAI Zhong-xiong LIU Sheng-cai |
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| Institution: | (Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou,Fujian 350002) |
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| Abstract: | In order to further study the influence factors of cotyledon regeneration system of in vitro Capsicum annuum L. , ＇Lajiaowang＇ seeds were used to induce in vitro plantlets（Capsicum annuum L. ）. The differences of adventitious buds induction were compared among the different concentrations of 6-BA. In the meanwhile, the effects of the different concentrations of GAa and 6-BA combinations on elongation were analyzed. The re- sults showed that the best medium for adventitious buds induction by hypocotyls was MB5＋0.8mg·L^-1IAA＋8.0mg·L^-16-BA＋4.0mg·L^-1AgNO3. The suitable medium for the adventitious buds elongation was MB5＋0.8mg·L^-1IAA＋5.0mg·L^-16-BA＋2.0mg·L^-1GA3＋4.0mg·L^-1AgNO3. |
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| Keywords: | Capsicum annuum L cotyledon adventitious buds induction elongation |
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