| 黄瓜种传镰刀菌粗毒素检测及其致害作用 |
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| 引用本文： | 蒋荷,郑慧慧,曹莎,吴学宏,黄俊生.黄瓜种传镰刀菌粗毒素检测及其致害作用[J].中国农业大学学报,2013,18(3):101-107. |
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| 作者姓名： | 蒋荷 郑慧慧 曹莎 吴学宏 黄俊生 |
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| 作者单位： | 1. 中国农业大学农学与生物技术学院/农业部植物病理学重点实验室,北京,100193
2. 中国热带农业科学院环境与植物保护研究所,海南儋州,571737 |
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| 基金项目： | 公益性行业(农业)科研专项经费项目资助 |
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| 摘 要： | 为检测黄瓜种传镰刀菌产生的粗毒素中镰刀菌酸含量及其对种子和幼苗的致害作用,采用乙酸乙酯和乙醚萃取法提取其中的镰刀菌酸并利用HPLC法进行定量分析,通过粗毒素处理黄瓜种子及幼苗根部测定其对种子发芽的影响和幼苗的致枯萎作用.结果表明:供试7株种传镰刀菌SC-CQ-P、HLJ-XC-S、LN-ZN203-S、GD-HY-S、HN-XH-S、LN-ZN6-S和HDTS-R粗毒素中都检测到镰刀菌酸,质量浓度分别为3574.71、866.43、291.10、123.32、856.13、358.27和327.71 μg/mL.种传镰刀菌粗毒素对黄瓜种子发芽具有一定的抑制作用；粗毒素浸渍黄瓜幼苗根部导致幼苗枯萎,并且其对幼苗的致枯萎作用随着镰刀菌酸浓度的升高和处理时间的延长而加重.
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| 关 键 词： | 黄瓜 种传镰刀菌 粗毒素 镰刀菌酸 HPLC |
| 收稿时间： | 2012/8/9 0:00:00 |
Study on the detection and harmful effect of crude toxin producedby cucumber seedborne Fusarium species |
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| JIANG He,ZHENG Hui-hui,CAO Sh,WU Xue-hong and HUANG Jun-sheng.Study on the detection and harmful effect of crude toxin producedby cucumber seedborne Fusarium species[J].Journal of China Agricultural University,2013,18(3):101-107. |
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| Authors: | JIANG He ZHENG Hui-hui CAO Sh WU Xue-hong and HUANG Jun-sheng |
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| Institution: | College of Agronomy and Biotechnology/The Key Laboratory of Plant Pathology, Ministry of Agriculture, China Agricultural University, Beijing 100193, China;College of Agronomy and Biotechnology/The Key Laboratory of Plant Pathology, Ministry of Agriculture, China Agricultural University, Beijing 100193, China;College of Agronomy and Biotechnology/The Key Laboratory of Plant Pathology, Ministry of Agriculture, China Agricultural University, Beijing 100193, China;College of Agronomy and Biotechnology/The Key Laboratory of Plant Pathology, Ministry of Agriculture, China Agricultural University, Beijing 100193, China;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China |
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| Abstract: | |
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| Keywords: | cucumber seedborne Fusarium crude toxin fusaric acid HPLC |
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