| 梨遗传连锁图谱的构建及部分果实性状QTL的定位 |
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| 引用本文： | 韩明丽,刘永立,郑小艳,杨健,王龙,王苏珂,李秀根,滕元文.梨遗传连锁图谱的构建及部分果实性状QTL的定位[J].果树学报,2010(4). |
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| 作者姓名： | 韩明丽 刘永立 郑小艳 杨健 王龙 王苏珂 李秀根 滕元文 |
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| 作者单位： | 浙江大学园艺系;农业部园艺植物生长发育与品质调控重点开放实验室;中国农业科学院郑州果树研究所; |
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| 基金项目： | 国家863计划项目(2006AA100108); 浙江省科技计划项目(2008C32011) |
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| 摘 要： | 利用八月红梨和砀山酥梨杂交得到的F1代实生苗为作图群体,应用Joinmap3.0作图软件,构建了一张包含61个苹果EST-SSR标记、68个苹果SSR标记、49个梨SSR标记、1个RAPD标记和3个质量性状标记,共182个标记并分属于19个连锁群的梨遗传连锁图谱,图谱总长度为982cM,标记间平均图距5.4cM。控制果皮红色的基因RFS位于LG3连锁群上,与最近的分子标记MES93-264p的遗传距离分别为9cM。控制果锈存在的基因FR、控制萼片脱落性状的基因AS分别位于LG16连锁群和LG12连锁群上。采用区间作图法,检测到与果实可溶性固形物含量、单果质量、横径和纵径等性状连锁的QTL位点21个(LOD≥2.5),其中主效QTL位点6个(LOD≥3.5),与果实性状相关QTL位点多集中在LG7和LG11连锁群上。
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| 关 键 词： | 梨 SSR EST-SSR 遗传图谱 QTL分析 果实性状 |
Construction of a genetic linkage map and QTL analysis for some fruit traits in pear |
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| HAN Ming-li,LIU Yong-li,ZHENG Xiao-yan,YANG Jian,WANG Long,WANG Su-ke,LI Xiu-gen,TENG Yuan-wen.Construction of a genetic linkage map and QTL analysis for some fruit traits in pear[J].Journal of Fruit Science,2010(4). |
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| Authors: | HAN Ming-li LIU Yong-li ZHENG Xiao-yan YANG Jian WANG Long WANG Su-ke LI Xiu-gen TENG Yuan-wen |
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| Institution: | HAN Ming-li1,LIU Yong-li1,ZHENG Xiao-yan1,YANG Jian2,WANG Long2,WANG Su-ke2,LI Xiu-gen2*,TENG Yuan-wen1* (1Department of Horticulture,Zhejiang University,The Key Laboratory of Horticultural Plant Growth,Development & Quality Improvement,Ministry of Agriculture,Hangzhou,Zhejiang 310029 China,2Zhengzhou Fruit Research Institute,CAAS,Zhengzhou,Henan 450009 China) |
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| Abstract: | A genetic linkage map was constructed from a F1 population comprising 91 seedlings derived from a cross between Bayuehong and Dangshansu using Joinmap3.0. The map consisted of 61 EST-SSR markers from apple,68 SSR markers from apple,49 SSR markers from pear,1 RAPD marker and 3 morphological traits which belonged to 19 linkage groups and spaned 982 cM with an average genetic distance of 5.4 cM. The locus RFS that controlled red fruit skin color was located on linkage group 3 and linked to the nearest markers ... |
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| Keywords: | Pear SSR EST-SSR Linkage map QTL analysis Fruit traits |
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