| 反义4CL1基因转化烟草调控木质素生物合成 |
| |
| 引用本文： | 杨雪萍,陆海,陈雪梅,蒋湘宁.反义4CL1基因转化烟草调控木质素生物合成[J].北京林业大学学报,2003,25(3):1-5. |
| |
| 作者姓名： | 杨雪萍 陆海 陈雪梅 蒋湘宁 |
| |
| 作者单位： | 北京林业大学生物科学与技术学院 |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划);G1999016005; |
| |
| 摘 要： | 将控制木质素生物合成的关键限速酶 (香豆酸CoA连接酶 ,4CL)基因 4CL1反向插入植物表达载体pBI12 1构建表达载体 ,通过农杆菌叶盘法介导转化烟草 ,获得一批转基因抗性植株 .经PCR检测 ,证明 4CL1基因已成功反义转入到烟草植株中 .通过对转基因植株和对照植株的纤维素和木质素含量的对比分析 ,发现反义 4CL1基因转化植株的纤维素含量比对照提高了 11 4 % ,而木质素含量比对照降低了 19 1% .
|
| 关 键 词： | 反义4CL1基因 转基因烟草 木质素 纤维素 |
| 修稿时间： | 2002年10月23日 |
Anti-sense 4CL1 changes the content and ratio of lignin and cellulose in transformed tobacco plantlets |
| |
| Yang Xueping,Lu Hai,Chen Xuemei,Jiang Xiangning. School of Biological Science and Biotechnology,Beijing For. Univ.,P. R. China..Anti-sense 4CL1 changes the content and ratio of lignin and cellulose in transformed tobacco plantlets[J].Journal of Beijing Forestry University,2003,25(3):1-5. |
| |
| Authors: | Yang Xueping Lu Hai Chen Xuemei Jiang Xiangning School of Biological Science and Biotechnology Beijing For Univ P R China |
| |
| Institution: | Yang Xueping,Lu Hai,Chen Xuemei,Jiang Xiangning. School of Biological Science and Biotechnology,Beijing For. Univ.,100083,P. R. China. |
| |
| Abstract: | The anti sense 4CL1 expression vector pBIFA1 was constructed by anti inserting 4 coumarate:CoA ligase (4CL1) gene into binary vector pBI121 The construction was transferred into tobacco by Agrobacterium mediated transformation method. Regenerated tobacco plantlets were verified by PCR, which indicated that the 4CL1 gene was integrated into the genomes of transformed plantlets. The lignin content of transgenic plantlets reduced by 19 1% compared to the control, while the cellulose content increased by 11 4%. |
| |
| Keywords: | anti sense 4CL1 gene transgenic tobacco lignin cellulose |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
