小尾寒羊MyoG外显子2和MyoD 5'侧翼区的多态性研究

采用PCR-SSCP技术分析了小尾寒羊肌细胞生成素Myo G基因外显子2和生肌决定基因Myo D 5'侧翼区的多态性,结果表明:Myo D 5'侧翼区在小尾寒羊中检测到2种基因型(BB、AB),BB和AB基因型频率分别为0.975 0和0.025 0,A和B等位基因频率分别为0.012 5和0.987 5;小尾寒羊的Myo G外显子2不存在多态性。对Myo D 5'侧翼区的BB基因型测序分析发现,其与牛的Myo D1基因序列(XM_592330.2)相比,发生了2处突变,分别是第960位发生了T→C突变、第972位发生了C→A突变。

Study on the Polymorphism of MyoG Exon 2 and MyoD 5' Flanking Region of Small Tail Han Sheep

The polymorphism of the MyoG exon 2 and the MyoD 5'' flanking region of the myoGenic decision gene were analyzed by PCR-SSCP in the Small Tail Han sheep breeds.The results showed that the MyoD 5''flanking region in the Small Tail Han sheep had 2 genotypes (BB,AB),BB and AB genotype frequencies were 0.975 0 and 0.025 0,A and B allele frequencies were 0.012 5 and 0.987 5.The polymorphism of MyoG exon 2 was not detected in the Small Tail Han sheep.Compared with the cattle''s MyoD1 gene sequence(XM_592330.2),BB genotype analysis of the MyoD 5'' flanking region revealed two mutations,the T→C mutation occurred at the 960th position,and the C→A mutation occurred at the 972th position.