以离子液体为介质生物胺的液相色谱测定

目的]寻求一种快速、简单、易推广的检测方法来测定食品和饮料中生物胺的含量。方法]以丹磺酰氯为衍生剂,室温离子液体作为萃取、富集、衍生介质来测定食品和饮料中生物胺的含量。结果]测定的过程包括柱前工作和色谱分离两部分,得出比较理想的衍生条件：丹磺酰氯溶液1.5ml,pH9.10缓冲液2.0ml,室温离子液体1.0ml,置于超声清洗器中超声30 min;超声后提取离子液注入HPLC,用UV检测仪（λ=254.16nm）可以使6种生物胺在30min内被分离出来。该方法将衍生、萃取和富集3个步骤集于一体,大大地简化了试验,离子液体可以有效地促进分离,稳定性良好,且所建立的方法线性范围良好。结论]测定食品和饮料中生物胺的含量,以丹磺酰氯为衍生剂,室温离子液体作为萃取、富集、衍生介质是一种快速、简单、易推广的检测方法。

Determination of the Biogenic Amines in the Media of Ionic Solution with the Method of Liquid Chromatography

Objective]The testing method of the biogenic amines(BAs) in food and beverage was developed.Method]The content of BAs in the food and beverage was tested with the dansly chloride as derivatization agent and the room temperature ionic liquid(RILT) as the medium of extraction,enrichment and derivatization.Result]The testing process included the work of pre-column and chromatographic separation and the ideal condition of derivative was as follows:the dansyl chloride solution,1.5 ml;the buffer(pH 9.10),2.0 ml and the RILT,1.0 ml,which was placed in ultrasonic cleaner for 30 minutes'treatment of ultrasound.The extracted RILT after ultrasonic treatment was injected into the HPLC and six types of BAs were separated within 30 minutes with UV detector(λ =254.16 nm).Three procedures:derivatization,extraction and enrichment were combined in the testing method,which was greatly simple.The ionic solution could effectively promote the separation with good stability and the method was also with good liner range.Conclusion]This method was quick,simplicity and easy to be operated.