凡纳滨对虾内源优势腐败菌的分离和鉴定

为了确定凡纳滨对虾在冷藏条件下内源优势腐败菌的种类,实验采用了细菌分离纯化法,将分离得到的内源腐败菌菌株进行分解蛋白质对比实验,筛选出分解蛋白质能力较强的菌株,进一步采用细菌自动生化系统鉴定法以及16S rRNA序列测定法进行鉴定,实验结果显示凡纳滨对虾在冷藏条件下的内源优势腐败菌是短杆菌属细菌,可以通过控制短杆菌属细菌的方法进行研究对虾内源保鲜技术。细菌自动生化系统鉴定结果的相似度为98.8%,16S rRNA序列鉴定结果的相似度为99.065%。

Separation and identification of endogenous dominant spoilage bacteria from Litopenaeus vannamei

Endogenous spoilage bacteria of Litopenaeus vannamei are the bacterial floras living in Litopenaeus vannamei body which cause the Litopenaeus vannamei spoilage.It is one of the most important factors which cause spoilage while the Litopenaeus vannamei are stored up and in transportation process.In order to find out the endogenous dominant spoilage bacteria of Litopenaeus vannamei under refrigeration,the bacteria separation is applied in experiment and therefore the endogenous spoilage bacteria are gained,and then the bacterial strains of high capability of decomposing protein were obtained after protein comparison experiment.Once more,auto-biochemical identification system and 16S rRNA sequencing are applied in experiment process.The result shows that the endogenous dominant spoilage bacteria of Litopenaeus vannamei are of Brevibacterium, and the semblance of auto-biochemical identification and 16S rRNA sequencing is 98.833% and 99.065% respectively.Researches show that the endogenous dominant spoilage bacteria of Litopenaeus vannamei under refrigeration are of Brevibacterium,and it is possible to research on endogenous freshness preservation technology of Litopenaeus vannamei by controlling Brevibacterium bacteria.