|
|||||
|
|
| 乳头管灌注脂多糖对兔血清酶活性的影响及病理组织学观察 | |
| 引用本文： | 曹随忠,邓华学,杨德英,杨永新,余树民,姚学萍,刘宗平.乳头管灌注脂多糖对兔血清酶活性的影响及病理组织学观察[J].动物医学进展,2011,32(4):67-70. |
| 作者姓名： | 曹随忠 邓华学 杨德英 杨永新 余树民 姚学萍 刘宗平 |
| 作者单位： | 1. 四川农业大学动物医学院,四川雅安625014;扬州大学兽医学院,江苏扬州225009 2. 重庆三峡职业学院农业与生物工程系,重庆,404155 3. 四川农业大学动物医学院,四川雅安,625014 4. 安徽省农业科学院畜牧兽医研究所,安徽合肥,230031 5. 扬州大学兽医学院,江苏扬州,225009 |
| 基金项目： | 中国博士后科学基金，四川省教育厅青年基金，教育部长江学者和创新团队发展计划创新团队项目 |
| 摘 要： | 探讨经乳头管灌注脂多糖(LPS)对兔血清酶活性的影响及乳腺病理组织学变化.12只泌乳兔(产后第7天)经乳头管灌注LPS(150 μg/kg体重)建立急性临床型乳房炎模型,在灌注前2 h,灌注后6、12、24、48、72 h以及5 d和7 d分别测定血清中乳酸脱氢酶(LDH)、髓过氧化物酶(MPO)、过氧化物酶(LP)及... |
| 关 键 词： | 兔 乳腺 脂多糖 乳酸脱氢酶 病理变化 |
Effect of LPS Administration Via Teat on Serum Enzyme Activities and Histopathology of Mammary Gland in Rabbits |
|
| CAO Sui-zhong,DENG Hua-xue,YANG De-ying,YANG Yong-xin,YU Shu-min,YAO Xue-ping,LIU Zong-ping.Effect of LPS Administration Via Teat on Serum Enzyme Activities and Histopathology of Mammary Gland in Rabbits[J].Progress In Veterinary Medicine,2011,32(4):67-70. | |
| Authors: | CAO Sui-zhong DENG Hua-xue YANG De-ying YANG Yong-xin YU Shu-min YAO Xue-ping LIU Zong-ping |
| Institution: | 1.College of Veterinary Medicine,Sichuan Agricultural University,Ya’an,Sichuan,625014,China;2.Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,Hefei,Anhui,230031,China;3.College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu,225009,China;4.Department of Agriculture and Bioengineering,Chongqing Three Gorges Vocational College,Chongqing,404155,China) |
| Abstract: | In order to investigate the effect of lipopolysaccharide(LPS) administration on activities of enzymes in rabbits,twelve lactating rabbits(7th day after parturition) were challenged by infusing LPS(150 μg/kg body weight) into the fourth mammary gland via the teat duct.At exactly 2 h before inoculation and 6,12,24,48,72 h after infusion LPS,the blood was taken to determine the activities of lactate dehydrogenase(LDH),myeloperoxidase(MPO),N-acetyl-β-D-glucosaminase(NAG) and lactoperoxidase(LP) in serum.At the same time,pathological lesions of mammary gland tissue were observed.The results showed that the activity of LDH,MPO,LP and NAG significantly increased within 6-72 h(P<0.05) after infusion LPS via teat canal.Histopatholgic examination results revealed that mammary gland had pathological lesions after 6h infusion LPS,but alveolar structures were still discernible,numbers of polymorphonuclears(PMNs) were present in alveoli.The mammary gland had more severe pathological lesion at 24 h after infusion,the normal alveolar structures were lost and infiltrated with numerous PMNs.Mammary gland tissue lesions regenerated after 72 h,returning to normal at 7 d.The LDH activity showed the maximum sensitivity to acute bovine mastitis which induced by LPS.The dynamic change of LDH activity was closely related to the mammary tissue damage.These results indicated that LDH could be as candidate indicator to detect the inflammation degree of mammary gland and earlier diagnosis of mastitis. |
| Keywords: | rabbits mammary gland lipopolysaccharide lactatedehydrogenase pathological changes |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |