| 文心兰茎尖诱导丛生芽高频率植株再生 |
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| 引用本文： | 叶秀仙,黄敏玲,吴建设,林兵,钟淮钦,陈源泉.文心兰茎尖诱导丛生芽高频率植株再生[J].福建农业学报,2009,24(2). |
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| 作者姓名： | 叶秀仙 黄敏玲 吴建设 林兵 钟淮钦 陈源泉 |
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| 作者单位： | 福建省农业科学院花卉研究中心,福建,福州,350013;福建省特色花卉工程技术研究中心,福建,福州,350013 |
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| 基金项目： | 国家科技支撑计划，福建省林业科技重大专项花卉专题项目，福建省科技计划重点项目，福建省农业科学院青年人才创新基金，福建省财政专项-福建省农科院科技创新团队建设基金 |
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| 摘 要： | 以文心兰茎尖为外植体,通过基本培养基(MS、1/2MS、1/2 N6)、植物激素(6BA、NAA)、培养条件(有机添加物、糖、培养物状态)等关键因子对文心兰丛生芽诱导、增殖、生根各培养阶段影响的试验,探讨了文心兰以丛生芽途径再生植株的关键技术.结果表明:茎尖诱导丛生芽较适培养基配方为1/2 MS+6BA 2.0 mg·L-1 +NAA 0.1 mg·L-1,诱导率为84.6%;丛生芽在MS+6BA 2.0 mg·L-1 +NAA 0.1 mg·L-1 +蔗糖30 g·L-1培养基中增殖效果较好,增殖系数为5.5;株高2.0～3.0 cm的试管苗是丛生芽增殖的最佳培养材料;生根培养基适宜配方为1/2MS+IBA0.5 mg·L-1 +苹果汁100 g·L-1 +活性碳0.5 g·L-1 +蔗糖20 g·L-1,生根率为100%.
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| 关 键 词： | 文心兰 丛生芽 植株再生 组织培养 |
Induced high-frequency plant regeneration of oncidium using its multiple shoots |
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| YE Xiu-xian,HUANG Min-ling,WU Jian-she,LIN Bing,ZHONG Huai-qin,CHEN Yuan-quan.Induced high-frequency plant regeneration of oncidium using its multiple shoots[J].Fujian Journal of Agricultural Sciences,2009,24(2). |
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| Authors: | YE Xiu-xian HUANG Min-ling WU Jian-she LIN Bing ZHONG Huai-qin CHEN Yuan-quan |
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| Institution: | 1.Flower Research Center;Fujian Academy of Agricultural Science;Fuzhou;Fujian 350013;2.Fujian Engineering Research Center for Characteristic Flowers;Fujian 350013 |
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| Abstract: | Shoot tips of Oncidium were used as the explants to study the effect of some key factors on the induction,propagation and rooting of multiple shoots in Oncidium.The factors studied included the culture media(i.e.,MS,1/2MS and 1/2 N6),phytohormone(i.e.,6-BA and NAA) application and culture conditions(i.e.,organic additives,sugar and culture stages).From the results it was hoped that the core technology of Oncidium regeneration using its multiple shoots could be established.It was found that(a) the medium con... |
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| Keywords: | Oncidium multiple shoots plant regeneration tissue culture |
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