| 应用PCR技术从牛肌肉组织中进行性别鉴定 |
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| 引用本文： | 李天俊,郑丽萍,段县平.应用PCR技术从牛肌肉组织中进行性别鉴定[J].天津农学院学报,2001,8(4):22-23,46. |
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| 作者姓名： | 李天俊 郑丽萍 段县平 |
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| 作者单位： | 天津农学院,生化实验室, |
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| 摘 要： | 应用牛肌肉组织X和Y染色体上的成釉蛋白基因第五内含因子的DNA序列，设计了一对特异性PCR引物，通过PCR扩增，分别从雌性和雄性牛肌肉组织中扩增出不同的条带，从而达到性别鉴定的目的。具有较高的应用价值。
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| 关 键 词： | PCR 牛 肌肉组织 性别鉴定 成釉蛋白基因 |
| 文章编号： | 1008-5394(2001)04-0022-03 |
Sex Diagnosis with Beef Muscular Tissue Through PCR Technology |
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| LI Tian jun,ZHENG Li ping,DUAN Xian ping.Sex Diagnosis with Beef Muscular Tissue Through PCR Technology[J].Journal of Tianjin Agricultural College,2001,8(4):22-23,46. |
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| Authors: | LI Tian jun ZHENG Li ping DUAN Xian ping |
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| Abstract: | DNA sequence which lies in the fifth intron of sex chromosome protein gene in beef muscular tissue has been used to design a pair of special PCR primer.Through PCR,different stripes can be expanded from male or female beef muscular tissue to diagnose sex and it is of great practical values |
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| Keywords: | PCR beef muscular tissue sex diagnosis |
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