猪粪中洛克沙胂的HPLC检测及排泄规律

在建立猪粪中洛克沙胂HPLC检测方法基础上,选用24头育肥猪,分别混饲给予0、25、50、100 mg/kg洛克沙胂,混饲给药后不同时间采集粪样,以高效液相色谱法测定其中洛克沙胂质量浓度,了解洛克沙胂混饲给药后在猪体内的排泄情况,然后从江苏和山东省15个使用洛克沙胂的集约化猪场采集150头猪的粪样,调查猪粪样中的洛克沙胂含量。结果表明,所建立的猪粪中洛克沙胂HPLC检测方法的平均回收率为82.09%~84.03%,变异系数为2.92%~5.45%,检测限为0.05 mg/kg,定量限为0.1 mg/kg;以不同剂量混饲给药后,洛克沙胂在粪中排泄量在36~48 h达峰,峰质量浓度分别为12.31、22.52、34.78 mg/kg,猪粪中检测不到洛克沙胂的时间分别为72、108、132 h;所调查150个粪样中洛克沙胂平均质量浓度为23.13 mg/kg。

HPLC determination of roxarsone and its elimination in feces of swine

A high performance liquid chromatography (HPLC) method was developed for determination of roxarsone levels in feces of swine. The limit of detection and limit of quantitation of roxarsone in feces of swine were 0.05 mg/kg and 0.1 mg/kg, respectively. The mean recoveries of roxarsone ranged from 82.09%-84.03%. Coefficients of var-iation ranged from 2.92%-5.45%. Twenty-four healthy changmei crossbreed pigs were randomly divided into 4 groups and were fed with feed containing roxarsone (0,25,50 and 100 mg/kg). Then, the feces were collected and analysed by HPLC at 0 to 120 h after administered. The excreted amount of roxarsone reached a peak at 36-48 h af-ter feeding,with a peak concentrarion of 12.31,22.52 and 34.78 mg/kg,respectively. Then,the amount of roxarsone in the feces reduced gradually,and roxarsone was undetectable in swine feces within 72,108 and 132 h after feeding, respectively. In 150 samples of swine feces collected from fifteen swine farms in Shandong and Jiangsu province, the average concentration of roxarsone in pig feces was 23.13 mg/kg.