人参遗传多样性的SSR分析

目的]分析吉林省5个人参产区人参（Panax ginseng C.A.Mey）种质资源存在的差异性,为参农异地引种提供理论依据。方法]利用SSR技术对吉林省5个人参产区9个人参类型的DNA进行检测,并利用DPS9.50专业版软件进行相异系数计算,用Nei-Li公式和UPGMA法构建聚类树状图。结果]从25对引物中筛选出16对多态性引物用于扩增,共扩增出181条条带,其中,多态性条带117条,多态性64.7%。结论]人参种质资源在分子水平上存在较大差异;人参不同类型间的差异主要由其遗传因素决定,但也与环境因素有关。

SSR Analysis of the Genetic Diversity of Panax ginseng

Objective] The differences among the ginseng germplasm resources in five different producing regions of Jilin Province were analyzed,so as to provide theoretical basis for the introduction of ginseng from other places. Method] By using simple sequence repeat ( SSR),nine ginseng materials of five districts in Jilin were used for genomic polymorphic analysis. The dissimilarity coefficient was calculated by professional software DPS 9.50. And the dendrogram was made by using Nei-Li formula and UPGMA. Result] 16 pairs of polymorphic primers were selected from 25 pairs of primers for the amplification. 181 bands were amplified and 117 bands were polymorphic,with the polymorphic percentage of 64.7%. Conclusion] There were much genetic diversity at the molecular level among the germplasm resources of Panax ginseng actually. The differences between the tested ginseng varietal types were determined by the genetic factors. Furthermore,it was also affected by environmental factors.