| 白灵菇深层发酵条件的优化 |
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| 引用本文： | 孟俊龙,闫辉,杨杰,钟华,郭亮,充娜,常明昌.白灵菇深层发酵条件的优化[J].山西农业大学学报(自然科学版),2010,30(6). |
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| 作者姓名： | 孟俊龙 闫辉 杨杰 钟华 郭亮 充娜 常明昌 |
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| 基金项目： | 山西省科技基础条件平台项目，山西农业大学科技创新基金 |
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| 摘 要： | 为了优化白灵菇深层发酵的条件,以菌丝生物量为指标,对碳源、氮源、初始pH、接种量、装液量进行单因素试验,结果表明160 r.min-125℃培养条件下,白灵菇最佳液体培养基配方为玉米淀粉4%、米糠5%、KH2PO40.3%、MgSO40.1%、VB110 mg.L-1,最佳装液量为70 mL(250 mL三角瓶),最佳接种量为20%,初始pH为7～8。正交试验结果表明,影响白灵菇液体培养的主次因素依次为接种量、碳源、氮源、初始pH和装液量。
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| 关 键 词： | 白灵菇 深层发酵 菌丝生物量 |
Optimization of Submerged Culture Conditions of Pleurotus nebrodensis |
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| MENG Jun-long,YAN Hui,YANG Jie,ZHONG Hua,GUO Liang,CHONG Na,CHANG Ming-chang.Optimization of Submerged Culture Conditions of Pleurotus nebrodensis[J].Journal of Shanxi Agricultural University,2010,30(6). |
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| Authors: | MENG Jun-long YAN Hui YANG Jie ZHONG Hua GUO Liang CHONG Na CHANG Ming-chang |
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| Abstract: | The paper studied the optimization of submerged culture conditions of Pleurotus nebrodensis using the mycelia biomass as the test index.Monofactorial test revealed that mycelial biomass of Pleurotus nebrodensis grown in submerged culture was maximal under the following conditions:160 r·min-1 temperature 25 ℃,corn starch 4%,rice bran 5%,KH2PO4 0.3%,MgSO4 0.1%,VB1 10 mg·L-1,the best broth content was 70 mL(250 mL Erlenmeyer flask),the best inoculated amount was 20%,the initial pH was 7～8.Application of orthogonal experiment revealed that mycelial biomass were influenced significantly by inoculated amount,followed by carbon source,nitrogen source,initial pH,broth content. |
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| Keywords: | Pleurotus nebrodensis Submerged fermentation Mycelial biomass |
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