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| 翦股颖属植物基因组DNA提取方法的比较 | |
| 引用本文： | 于得水,刘金荣,周玉雷,裴彩霞,陈平.翦股颖属植物基因组DNA提取方法的比较[J].广东农业科学,2009(12):164-167. |
| 作者姓名： | 于得水 刘金荣 周玉雷 裴彩霞 陈平 |
| 作者单位： | 1. 甘肃农业大学草业学院/教育部草业生态系统重点实验室/中-美草地畜牧业可持续发展研究中心,甘肃,兰州,730070;仲恺农业工程学院农业与园林学院,广东,广州,510225 2. 兰州大学草地农业科技学院/农业部草地农业生态系统学重点开放实验室,甘肃,兰州,730020 3. 仲恺农业工程学院农业与园林学院,广东,广州,510225 |
| 基金项目： | 广东省科技攻关项目，广州市科技攻关项目，广东省农业标准项目，珠海市科技攻关项目，深圳市深绿圃林技术实业有限公司项目，仲恺农业工程学院博士启动项目 |
| 摘 要： | 采用试剂盒法、高盐低pH法、改良高盐低pH法、吸附柱法、SDS法、改良CTAB法,分别对翦股颖属植物品种Penncross、Seaside、PennA-4、粤选1号和新品系粤选10号的基因组DNA进行提取并经DNA电泳检测、蛋白分析仪分析和RAPD分析.结果表明:改良的CTAB法、改良高盐低pH法能够分离出产率高、纯度高、质量好的DNA,而吸附柱法的DNA产率偏低、但纯度较高,3种方法均能扩增出清晰的条带. |
| 关 键 词： | 翦股颖 提取方法 |
Comparison of DNA extraction methods of Agrostis genome |
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| YU De-shui,LIU Jin-rong,ZHOU Yu-lei,PEI Cai-xia,CHEN Ping.Comparison of DNA extraction methods of Agrostis genome[J].Guangdong Agricultural Sciences,2009(12):164-167. | |
| Authors: | YU De-shui LIU Jin-rong ZHOU Yu-lei PEI Cai-xia CHEN Ping |
| Institution: | YU De-shui1,2,LIU Jin-rong3,ZHOU Yu-lei2,PEI Cai-xia1,CHEN Ping2(1.College of Pratacuhural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education/ Sino-U.S.Centers for Grazingland Ecosystem Sustainability,Lanzhou 730070,China,2.College of Agriculture and Landscape,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,3.College of Pastoral Agriculture Science and Technology,Lanzhou University/Key Laboratory of Grassland Agro-Ecosystems,Ministry... |
| Abstract: | Six methods included kit,high salt low pH,modified high salt low pH,column adsorption,SDS(Sodium dodecyl sulfate),modified CTAB(cetyltrimethyle ammonium bromide) were compared for extracting DNA from Agrostis varieties: Penncross,Seaside,PennA-4,Yuexuan No.1 and new strain Yuexuan No.10.The results showed that modified high salt low pH,modified CTAB and column adsorption methods could extract DNA of high-quality and high-integrity,as determined by agarose gel electrophoresis and UV-spectroscope.While the DN... |
| Keywords: | DNA RAPD Agrostis DNA extraction method RAPD |
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