| 南瓜离体培养及植株再生的研究 |
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| 引用本文： | 余义和,李桂荣,王新娟,周明,刘玉博.南瓜离体培养及植株再生的研究[J].安徽农业科学,2006,34(20):5180-5181. |
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| 作者姓名： | 余义和 李桂荣 王新娟 周明 刘玉博 |
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| 作者单位： | 河南科技学院园林学院园艺植物组织培养研究室,河南,新乡,453003 |
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| 摘 要： | 研究了不同外植体、消毒方法、激素水平等对南瓜离体培养及植株再生的影响。结果表明:以子叶作为外植体可以通过愈伤组织途径之后形成再生植株;而以子叶节作为外植体可以直接诱导不定芽形成再生植株。诱导不定芽的最佳培养基组合为MS+1.0 mg/L6-BA+0.5 mg/L NAA;诱导愈伤组织的最佳培养基组合为MS+0.5mg/L 6-BA+0.5mg/L 2,4-D;诱导南瓜不定根的最佳培养基组合为MS+1.0 mg/L6-BA+0.5 mg/LIAA。
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| 关 键 词： | 南瓜 激素 子叶 组织培养 |
| 文章编号： | 0517-6611（2006）20-5180-02 |
| 收稿时间： | 05 11 2006 12:00AM |
| 修稿时间： | 2006-05-11 |
Study on Pumpkin Culture in Vitro and Regeneration |
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| YU Yi-he, et al.Study on Pumpkin Culture in Vitro and Regeneration[J].Journal of Anhui Agricultural Sciences,2006,34(20):5180-5181. |
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| Authors: | YU Yi-he |
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| Institution: | Horticulture Plant Tissue Culture Institute of College of Landscape, Henan Institute of Science and Technology, Xinxiang, Henan 453003 |
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| Abstract: | Adventitious shoots were regenerated from cotyledon and cotyledon nodes of pumpkin.The result showed that cotyledon being used as explants,the plant regeneration can obtain from the way of growing callus,and cotyledon nodes being used as explants,plant regeneration can obtain direct from inducing adventitious bud.At the same time,the result showed that the optimum medium for adventitious bud was MS medium supplement with 1 mg/L 6-BA 0.5 mg/L NAA.The callus induced in the medium of MS medium supplement with 0.5 mg/L 6-BA 0.5 mg/L 2,4-D was best;and the adventitious root was induced on MS medium supplemented with 1 mg/L 6-BA 0.5 mg/L IAA. |
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| Keywords: | Hormone Pumpkin Cotyledon Tissue culture |
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