二乙基己烯雌酚对成年仓鼠附睾肥大细胞数量分布的影响

成年雄性仓鼠经皮下连续注射二乙基己烯雌酚(DES)7 d后,用改良甲苯胺蓝染色法(MTB)和阿尔新蓝番红染色法(AB S)研究DES对附睾肥大细胞的形态大小、类型和数量分布的影响。结果表明:仓鼠附睾肥大细胞为结缔组织型肥大细胞,多分布于附睾头与附睾尾的被膜和间质中。试验组的肥大细胞常见于附睾管近旁,多呈脱颗粒状。虽然试验组与对照组的肥大细胞大小相近,但试验组的肥大细胞数量增多,尤其是附睾尾间质中肥大细胞增加明显(P<0.01)。

Study on the Number and Distribution of Epididymis Mast Cells in DES-treated Adult Hamster

After administrating DES (diethylstilbestrol) by subcutaneous injections for 7 days, the changes in the morphology, distribution and number of mast cells in adult hamster epididymis caput and caudal were studied by using modified toluidine blue (MTB) staining, and cytochemical subsets of mast cells were identified by alcian blue-safranin O (AB-S) staining. The relationship of the number of epididymis mast cells between the morphology changes of epididymis caudal during DES-treated were explored. The results showed: Epididymis mast cells were belong to connective mast cells (CTMC), which distributed in the capsule and interstitium of epididymis caput and caudal. Some mast cells localized nearby epididymis tubular, and degranule mast cells could be seen in the epididymis caput and caudal in DES-treated hamster. Although mast cells area have no obvious difference in both DES-treated and control groups, but the number of epididymis caudal mast cells were significant increased in DES-treated (P<0.01), particularly in epididymis caudal.