焦锑酸钾沉淀法定位Ca~(2+)在梨生理研究中的应用

介绍了应用焦锑酸钾沉淀法定位Ca2＋的方法，以及在梨生理研究中的具体步骤和应用效果。结果表明：在固定液中加焦锑酸钾处理进行制样，梨树不同器官或组织细胞中的自由态Ca2＋，都能生成焦锑酸钙在原部位沉淀，经染色后在电子显微镜下呈现出黑色颗粒，而固定液中不加焦锑酸钾处理的则没有这种颗粒。再进一步用Ca2＋的专一性鳌合物EGTA进行处理，检查这些黑色颗粒是否消失，以证实黑色颗粒就是Ca2＋，从而可对Ca2＋进行定位标记。在不同的组织细胞及同一组织细胞在不同的生理时期，其被标记的Ca2＋密度和场所是不同的，可通过其动态变化来探讨它的作用机理。还对用此方法定位Ca2＋的注意事项进行了讨论。

Localization of Ca(2+) by Antimonite Precipitation Technique and Its Application to Pear Physiological Research

The method of localising Ca2＋ by antimonite precipitation technique the detailed procedure and effects of its application to fruit physiological research were presented in this paper． The results showed that with treatment of the fixative solution added with antimonite the free Ca2＋ in pear different organs and tissues could be changed into antimonite calcium and precipitated in situ which after being stained appeared as black particles under electronic microscope whereas such particles could not be observed in the treatment without antimonite． Furthermore the samples were treated with EGTA a specific reagent in order to see whether the black particles would disappear and to confirm that the black particles were Ca2＋ so that Ca2＋ could be localised and marked． In different tissues and during different physiological periods of the same tissue the density and site of the marked Ca2＋ were different which could be used to study the mechanism of Ca2＋ by means of its fluctuation． The problems to which we should pay attention when the localization of Ca2＋ was conducted by this method were also discussed．