刺五加不同药用部位中槲皮素鼠李糖苷的含量比较

目的]测定刺五加不同药用部位中槲皮素鼠李糖苷含量,并对结果进行比较,为刺五加合理利用及质量评价提供科学依据。方法]采用高效液相色谱法测定刺五加的根、茎、叶、花和果中槲皮素鼠李糖苷含量。色谱条件为:大连江申Nucleosil C18(4.6 mm×150 mm,5μm)色谱柱,流动相为无水甲醇-水(40∶60,V/V),流速为1.1 ml/min,检测波长为370 nm,柱温为30℃,进样量为10μl。结果]刺五加各药用部位中槲皮素鼠李糖苷的含量大小顺序为花蕾叶果茎根。其中,花蕾中槲皮素鼠李糖苷含量最高,为4.43%;其次为叶(0.61%);根中的最低,为0.01%。结论]该方法操作简便、快速、准确,可用于刺五加不同部位中的槲皮素鼠李糖苷的含量比较。

Comparisons of Quercetin-rhamnoside Contents among Different Medicinal Parts of Acanthopanax senticosus(Rupr.et Maxim.) Harms

Objective] To determine the content of quercetin-rhamnoside in different medicinal parts of Acanthopanax senticosus(Rupr.et Maxim.) Harms and compare them,so as to provide scientific basis for rational utilization and quality evaluation of A.senticosus.Method] HPLC was used to determine the contents of quercetin-rhamnoside in root,stem,leaf,flower and fruit.HPLC analysis was performed on Dalian Jiangshen Nucleosil C18 column(4.6 mm×150 mm,5 μm) with mobile phase of methyl alcohol-water(V/V,40∶ 60) and flow rate of 1.1 ml/min.The other conditions were: detection wavelength of 370 nm,column temperature of 30 ℃,sampling amount of 10 μl.Result] Different medicinal parts of A.senticosus were arranged in order of quercetin-rhamnoside content: flower bud > leaf > fruit > stem > root.Content of quercetin-rhamnoside was the highest in flower bud(4.43%),followed by leaf(0.61%),and the least in root(0.01%).Conclusion] This method was simple,quick and accurate,and can be used for analysis of quercetin-rhamnoside in different medicinal parts of A.senticosus.