| 梨矮化基因pcDw的SSR标记定位 |
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| 引用本文： | 田义轲,王彩虹,贾彦利,王亮,戴洪义.梨矮化基因pcDw的SSR标记定位[J].果树学报,2008,25(3):404-407. |
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| 作者姓名： | 田义轲 王彩虹 贾彦利 王亮 戴洪义 |
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| 作者单位： | 青岛农业大学园艺学院,山东青岛,266109 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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山东省自然科学基金
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山东省青岛市科技攻关项目 |
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| 摘 要： | 以矮化梨(Pyrus communis L.)与茌梨(P.bretschneideri Rehd.)的F1杂交分离群体共110个单株(3a生,矮化型和正常型各55株)为试材,对来自西洋梨的矮化型突变基因pcDw进行了SSR分子标记研究。用分离群体分组分析法(Bulked Segregant Analysis,BSA),通过对源自梨、苹果和桃基因组的共40对SSR(Simple Sequence Repeat)引物的筛选,获得了一个与pcDw基因连锁距离为9.3cM的SSR标记KA14210,由此将该基因定位到了梨品种Barlett遗传图谱的第16连锁群上。
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| 关 键 词： | 梨 矮化性状 pcDw基因 SSR标记 图谱定位 |
| 文章编号： | 1009-9980(2008)03-404-04 |
| 修稿时间： | 2007年7月16日 |
Location of a pear dwarf gene pcDw by SSR marker |
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| TIAN Yi-ke,WANG Cai-hong,JIA Yan-li,WANG Liang,DAI Hong-yi.Location of a pear dwarf gene pcDw by SSR marker[J].Journal of Fruit Science,2008,25(3):404-407. |
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| Authors: | TIAN Yi-ke WANG Cai-hong JIA Yan-li WANG Liang DAI Hong-yi |
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| Abstract: | The F1 segregation population, total of 110 (3-year-old) seedlings derived from the cross Aihuali (Pyrus communis L.) ×Chili (P. bretschneideri Rehd.) was tested for identifying SSR molecular markers linked to a pear dwarf gene pcDw, which produced by a mutation of P. communis. Altogether, 40 pairs of SSR primers originating from pear, apple and peach genome were screened by bulked segregant analysis (BSA). Finally, a SSR marker, KA14210, which linked to pcDw gene at a distance of 9.3 cM, was acquired. By this marker, pcDw gene was located on LG16 of the genetic map of pear variety Barlett. |
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| Keywords: | Pear Dwarf trait pcDw gene SSR marker Map location |
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