| 虾夷扇贝胚胎及早期幼虫酯酶和过氧化物酶的组织化学研究 |
| |
| 引用本文： | 冉镇,孙虎山,王宜艳,韩强,曾鹏.虾夷扇贝胚胎及早期幼虫酯酶和过氧化物酶的组织化学研究[J].水产科学,2007,26(4):204-206. |
| |
| 作者姓名： | 冉镇 孙虎山 王宜艳 韩强 曾鹏 |
| |
| 作者单位： | 1. 鲁东大学,生命科学学院,山东,烟台,264025
2. 鲁东大学,生命科学学院,山东,烟台,264025;山东师范大学,生命科学学院,山东,济南,250014 |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| |
| 摘 要： | 采用组织化学的方法对虾夷扇贝从卵细胞到D形面盘幼虫内的酯酶(NSE)和过氧化物酶(POD)进行定性定位研究。试验结果表明,NSE和POD均广泛存在于虾夷扇贝的胚胎和幼虫中,自卵细胞开始就已存在。从卵细胞到原肠胚均呈比较均一的NSE强阳性或POD阳性。在担轮幼虫期,中央的内脏团原基呈NSE极强阳性或POD强阳性,其余细胞则呈NSE强阳性或POD阳性。D形面盘幼虫期,外套膜和内脏团呈NSE极强阳性或POD强阳性,其余部位则呈NSE强阳性或POD弱阳性。
|
| 关 键 词： | 虾夷扇贝 胚胎 幼虫 酯酶 过氧化物酶 组织化学 |
| 文章编号： | 1003-1111(2007)04-0204-03 |
| 修稿时间： | 2006-05-24 |
Histochemical Observation of Esterase and Peroxidase in Embryo and Early Larva Stage of Scallop Patinopecten yessoensis |
| |
| RAN Zhen,SUN Hu-shan,WANG Yi-yan,HAN Qiang,ZENG Peng.Histochemical Observation of Esterase and Peroxidase in Embryo and Early Larva Stage of Scallop Patinopecten yessoensis[J].Fisheries Science,2007,26(4):204-206. |
| |
| Authors: | RAN Zhen SUN Hu-shan WANG Yi-yan HAN Qiang ZENG Peng |
| |
| Institution: | 1. College of Life Science, Ludong University, Yantai 264025, China; 2. College of Life Science, Shandong Normal University, Jinan 250014, China |
| |
| Abstract: | The non-special esterase(NSE) and the peroxidase(POD) were studied in the embryo and early larva of scallop Patinopecten yessoensis by histochemical localization method.The results showed that both NSE and POD existed in the egg,embryo or early larva of the scallop.The distribution of NSE and POD were relatively even in the embryo from the oocyte to the gastrula and the embryo showed very strong positive NSE and positive POD.The cells in the centre of the trochophore showed very strong positive NSE and strong positive POD.Most of the NSE and POD was located on the margin of mantle and visceral mass in veliger larvae. |
| |
| Keywords: | Patinopecten yessoensis embryo larva non-special esterase peroxidase histochemistry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
