| 平邑甜茶子叶和下胚轴离体再生不定芽的研究 |
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| 引用本文： | 刘霁菡,代红艳,赵恺,张志宏.平邑甜茶子叶和下胚轴离体再生不定芽的研究[J].东北农业大学学报,2011,42(10):90-94. |
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| 作者姓名： | 刘霁菡 代红艳 赵恺 张志宏 |
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| 作者单位： | 沈阳农业大学园艺学院,沈阳,110866 |
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| 基金项目： | 辽宁省高等学校创新团队项目(2008T177) |
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| 摘 要： | 为了建立高效的平邑甜茶离体再生体系,以平邑甜茶子叶和下胚轴为外植体,研究了不同外植体部位、暗培养时间、基本培养基、激素种类和浓度对不定芽再生的影响.结果表明,近轴端子叶的再生频率是远轴端子叶的3.1倍、下胚轴的3.7倍.在附加不同浓度的NAA、IBA与TDZ的SC培养基上,近轴端子叶的不定芽再生率达90％以上.在附加1...
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| 关 键 词： | 平邑甜茶 子叶 再生 不定芽 |
In vitro adventitious bud regeneration from cotyledon and hypocotyl of Malus hupehensis var.pingyiensis |
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| LIU Jihan,DAI Hongyan,ZHAO Kai,ZHANG Zhihong.In vitro adventitious bud regeneration from cotyledon and hypocotyl of Malus hupehensis var.pingyiensis[J].Journal of Northeast Agricultural University,2011,42(10):90-94. |
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| Authors: | LIU Jihan DAI Hongyan ZHAO Kai ZHANG Zhihong |
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| Institution: | LIU Jihan,DAI Hongyan,ZHAO Kai,ZHANG Zhihong(College of Horticulture,Shenyang Agricultural University,Shenyang 110866,China) |
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| Abstract: | To establish a highly efficient in vitro regeneration system of Malus hupehensis var.pingyiensis,the effects of types of explants,time of culture in darkness,basic medium,kind and concentration of plant growth regulator on adventitious bud regeneration from cotyledon and hypocotyl were studied.The regeneration rate of paraxial cotyledons was 3.1 times of that of abaxial cotyledons and 3.7 times of that of hypocotyls.On the SC medium supplemented with different combinations of NAA,IBA and TDZ,the regeneratio... |
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| Keywords: | Malus hupehensis var pingyiensis cotyledon regeneration adventitious bud |
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