| 蓝猪耳再生条件优化及不同外植体再生比较 |
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| 引用本文： | 李梅兰,康培婧,李洪清.蓝猪耳再生条件优化及不同外植体再生比较[J].山西农业大学学报(自然科学版),2005,25(1):34-37. |
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| 作者姓名： | 李梅兰 康培婧 李洪清 |
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| 作者单位： | 1. 华南师范大学,生命科学院,广东,广州,510631
2. 广东省,植物发育生物工程重点实验室,广东,广州,510631 |
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| 基金项目： | 广东省自然科学基金项目资助 (003062)，中国博士后科学基金 (2004036504) |
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| 摘 要： | 为了寻求一种最佳的蓝猪耳再生体系, 比较了叶片诱芽、诱愈及幼苗生根的几种培养基, 结果表明,1 /2MS+BA1mg/L+NAA0 1mg/L、MS+BA1mg/L+ 2 .4 D0 1mg/L和1 /2MS分别是诱芽、诱愈及生根的最佳培养基。不同外植体诱芽结果显示, 根、茎和叶片都可以诱导不定芽的产生, 但以叶片诱导的效率为最高。几种再生体系比较的结果表明, 蓝猪耳叶片诱芽的再生体系为最佳再生体系。
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| 关 键 词： | 蓝猪耳 再生 外植体 培养基 |
| 文章编号： | 1671-8151(2005)01-0034-04 |
| 修稿时间： | 2004年4月2日 |
Screening of the Best Regenerative Condition and Comparison of Different Explants in Regeneration of Torenia |
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| LI Mei-lan et al..Screening of the Best Regenerative Condition and Comparison of Different Explants in Regeneration of Torenia[J].Journal of Shanxi Agricultural University,2005,25(1):34-37. |
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| Authors: | LI Mei-lan |
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| Abstract: | In order to choose a best regeneration system, several medium used to induce shoot, callus and root were compared. The results showed that 1/2MS + BA 1 mg/L + NAA 0.1 mg/L, MS + BA 1 mg/L+ 2.4-D 0.1 mg/L and 1/2MS were the best media respectively. Shooting test carried out with different explants indicated that regeneration buds could be induced from roots, stem segments and leaves in suitable media and the leaf-explants were the most efficient. Comparative results among regeneration systems showed that the shooting regeneration system from leaf-explant was the best. |
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| Keywords: | Torenia Regeneration Explant Medium |
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