The Pig Lhx8 Gene: cDNA Cloning, Bioinformatic Analysis and Expression Level in Tissues and Preimplantation Embryos |
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作者单位: | College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095. P.R.China |
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基金项目: | This study was supported by the High Technology Research and Development Program of China (2006AA 10Z 136). |
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摘 要: | Evidence has shown in mouse that Lhx8 is a critical factor for maintenance and differentiation of the oocyte during early oogenesis. In the current paper, attempts were made to clone and characterize a gene encoding Lhx8 from pig. Rapid amplification of cDNA ends (RACE) gave rise to a full-length of Lhx8 which contained 1 681 bp nucleotides, with a complete open reading frame of 885 bp, encoding a 295 amino acid polypeptide. Homology search and sequence multialignment demonstrated that the deduced pig Lhx8 protein sequence shared a high identity with Lhx8 from other mammals, including several highly conservative motifs and amino acids. The phylogenetic tree of the LIM superfamily proteins has been constructed to reveal the evolutionary relationship of various species. RT-PCR analysis showed that the Lhx8 gene was expressed in gonad and immunity tissues. In preimplantation embryos, Lhx8 mRNA expression profiling using realtime PCR revealed that its mRNA levels were highest in 4-cell stage embryos and gradually decreased until the blastocyst stage.
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关 键 词: | cDNA克隆 mRNA水平 植入前胚胎 基因编码 免疫组织 生物信息 蛋白质序列 PCR分析 |
收稿时间: | 4 March 2009 |
The Pig Lhx8 Gene:cDNA Cloning,Bioinformatic Analysis and Expression Level in Tissues and Preimplantation Embryos |
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Authors: | FANG Wei LI Gui-qiang LI Mei-li WANG Wei XU Yin-xue |
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Institution: | College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095. P.R.China |
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Abstract: | Evidence has shown in mouse that Lhx8 is a critical factor for maintenance and differentiation of the oocyte during early oogenesis. In the current paper, attempts were made to clone and characterize a gene encoding Lhx8 from pig. Rapid amplification of cDNA ends (RACE) gave rise to a full-length of Lhx8 which contained 1 681 bp nucleotides, with a complete open reading frame of 885 bp, encoding a 295 amino acid polypeptide. Homology search and sequence multi-alignment demonstrated that the deduced pig Lhx8 protein sequence shared a high identity with Lhx8 from other mammals, including several highly conservative motifs and amino acids. The phylogenetic tree of the LIM superfamily proteins has been constructed to reveal the evolutionary relationship of various species. RT-PCR analysis showed that the Lhx8 gene was expressed in gonad and immunity tissues. In preimplantation embryos, Lhx8 mRNA expression profiling using real-time PCR revealed that its mRNA levels were highest in 4-cell stage embryos and gradually decreased until the blastocyst stage. |
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Keywords: | pig Lhx8 molecular cloning mRNA expression qRT-PCR preimplantation embryos |
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