| IBDV南京野毒株VP2结构蛋白基因克隆与表达 |
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| 引用本文： | 姜平,陈溥言.IBDV南京野毒株VP2结构蛋白基因克隆与表达[J].南京农业大学学报,1996,19(4):61-65. |
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| 作者姓名： | 姜平 陈溥言 |
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| 作者单位： | 南京农业大学畜禽传染病研究室 |
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| 基金项目： | 高等学校博士学科点专项科研基金 |
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| 摘 要： | 用微机辅助设计合成了一对引物，用于扩增传染性法氏囊病病毒中国地方株ＶＰ２基因片段。ＲＴ－ＰＣＲ增出－１３２１ｂｐ的目的的片段，分子杂交鉴定为ＶＰ２基因。
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| 关 键 词： | 病毒 结构蛋白 基因克隆 基因表达 IBD |
CLONING AND EXPRESSION OF VP2 STRUCTURAL PROTEIN GENE OF CHINESE FIELD VIRULENT IBDV IN E.COLI |
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| Jiang Ping,Chen Puyan and Cai Baoxiang.CLONING AND EXPRESSION OF VP2 STRUCTURAL PROTEIN GENE OF CHINESE FIELD VIRULENT IBDV IN E.COLI[J].Journal of Nanjing Agricultural University,1996,19(4):61-65. |
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| Authors: | Jiang Ping Chen Puyan and Cai Baoxiang |
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| Abstract: | A set of primers were designed to amplify the VP2 gene fragmnet of IBDV from China. A fragment of 1 321 bp was generated by RT PCR, resulting positive while detected by hybridization assay with VP2 nucleic acid probe. The pCVP21 was contructed by inserting the amplified VP2 gene fragment into expression vector pCYTEXP1, containing bacteriophage promoters P R and P L in tandem preceded by the cIts 857 repressor gene. Four colons, expressing VP2 protein, were selected by Dot ELISA with IBDV antiserum. SDS PAGE and Western blot analyses indicated that a 32 000 protein was expressed as expected. |
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| Keywords: | IBDV structure protein RT PCR gene clon gene expression |
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