甘肃枸杞属植物的RAPD分析

目的]研究甘肃枸杞属植物种间亲缘关系。方法]提取甘肃省内分布的枸杞属植物的基因组DNA,用RAPD方法检测其DNA多态性,并用UPGMA聚类法对其种间关系进行分析。结果]从80条10碱基的随机引物中筛选出了28条适用于枸杞属植物RAPD分析的引物。在6份种质材料中共扩增出了171条带,其中118条为多态性条带,多态性条带的比例为69.0%;遗传相似性系数在0.39～0.70之间,平均值为0.59。结论]聚类分析显示6份甘肃枸杞属种质材料中宁夏枸杞、北方枸杞和截萼枸杞亲缘较近,中国枸杞和新疆枸杞亲缘较近,黑果枸杞与其他种质间遗传距离较远。

Analysis of Wolfberry (Lycium L.) in Gansu by RAPD

Objective ] To study the genetic relationship among species in Lycium L. of Gansu Province. Method ] The genomic DNA was extracted from Lycium L. in Gansu Province and its polymorphism and genetic relationship were analyzed by RAPD and UPGMA cluster meth- od. Result] 28 primers were screened from 80 random primers of 10 bases for RAPD analysis of 6 samples. By using 28 primers, a total of 171 bands were obtained and 118 （69.0%） showed polymorphism. The genetic similarity coefficients were between 0.39 and 0.70 and the av- erage value was 0.59. Conclusion ] Cluster analysis indicated that the closer relationship was between L. chinense Mill. and L. dasystemum Pojark. , Lycium barbarum L. , L. chinese Mill. var. potaninii（Pojark. ）A. M. Lu and L. trunlcatum Y. C. Wang were clustered together. L. ru- thenicum Murr. is highly divergent from other species.